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undergraduate thesis
Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:653554
Bićanić, Anamarija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bićanić, A. (2010). Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:653554

Bićanić, Anamarija. "Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:653554

Bićanić, Anamarija. "Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:653554

Bićanić, A. (2010). 'Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 11 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:653554

Bićanić A. Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 December 11] Available at: https://urn.nsk.hr/urn:nbn:hr:159:653554

A. Bićanić, "Strukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:653554

Metadata
TitleStrukturne promjene dinukleotidnog mikrosatelitnog markera D17S855 gena BRCA1 u tumorima zametnih stanica testisa
Title (english)Structural changes of dinucleotide microsatellite marker D17S855 of BRCA1 gene in testicular germ cell tumors
AuthorAnamarija Bićanić
MentorReno Hrašćan (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2010-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Svrha rada bila je ispitati prisutnost gubitka heterozigotnosti (LOH) i mikrosatelitne nestabilnosti (MSI) u uzorcima tkiva od dvadeset pacijenata, od kojih je deset imalo seminomne, dok je ostalih deset imalo neseminomne tumore. Pritom smo koristili tehniku umnažanja DNA PCR-om intragenskog mikrosatelitnog markera D17S855 u genu BRCA1, te gel elektroforezu za iščitavanje rezultata. Na svim uzorcima pacijenata primjećena je informativna heterozigotnost, a kod tri od dvadeset uzoraka prisutna je mikrosatelitna nestabilnost u obliku skraćenog alela. Uočena MSI može biti dio ukupne genomske nestabilnosti tih tumora, iako su dobiveni samo preliminarni rezultati.
Abstract (english)
The aim of the study was to examine the presence of loss of heterozygosity (LOH) and microsatellite instabillity (MSI) in tissue samples from twenty patients, of whom ten had seminomal, while the other ten had nonseminomal tumors. Thereby, we used the PCR technique to amplify intragenic mikrosatellite marker D17S855 within BRCA1 gene and gel electrophoresis in order to visualize results and to draw conclusion. Informative heterozigosity was observed in all patient samples and in three of twenty samples, microsatellite instabillity is present in the form of shortened allel. Results are preliminary but observed MSI may be integral part of inherent genomic instability in those tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:653554
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-06-07 12:10:14