prikaz prve stranice dokumenta Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa
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undergraduate thesis
Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:275051
Melkić, Tina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Melkić, T. (2024). Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:275051

Melkić, Tina. "Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:275051

Melkić, Tina. "Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:275051

Melkić, T. (2024). 'Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:275051

Melkić T. Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:275051

T. Melkić, "Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:275051

Metadata
TitleAnaliza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa
Title (english)Analysis of genetic diversity and proteomic characterization of lactic acid bacteria isolated from sauerkraut brine
AuthorTina Melkić
MentorMartina Banić (mentor)
MentorKatarina Butorac (pomoć pri izradi, neposredni voditelj)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2024-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bakterije mliječne kiseline (BMK) naseljavaju različite dijelove ljudskog tijela i ekosustave poput biljaka, tla, mlijeka i mesa. Komercijalno se primjenjuju u prehrambenoj, kozmetičkoj, farmaceutskoj i kemijskoj industriji. U ovom radu izolirano je 30 sojeva BMK iz rasola tradicionalno proizvedenog kiselog kupusa. Izmjerene koncentracije DNA navedenih sojeva oscilirale su u rasponu od 89,19 ng/μL do 475,96 ng/μL, s prosječnom vrijednošću od 238,77 ± 89,18 ng/μL. Provedeno je genotipsko profiliranje pomoću RAPD-PCR analize te su dobiveni elektroforetski profili RAPD-PCR produkata uspoređeni hijerarhijskom klaster analizom s ciljem eliminacije istovjetnih bakterijskih izolata. Analizom dendrograma, ustanovljeno je da je 30 sojeva svrstano u 3 klastera, što ukazuje da su iz rasola kiselog kupusa izolirana tri genetički različita soja. SDS-PAGE analizom nije utvrđena prisutnost dominantnih proteinskih vrpci veličine oko 50 kDa, što ukazuje da sojevi izolirani u ovom radu najvjerojatnije ne proizvode S-proteine.
Abstract (english)
Lactic acid bacteria (LAB) inhabit various parts of the human body and ecosystems such as plants, soil, milk, and meat. They are commercially utilised in the food, cosmetic, pharmaceutical and chemical industries. In this work, 30 LAB strains were isolated from the brine of traditionally produced sauerkraut. The measured DNA concentrations of these strains ranged from 89.19 ng/μL to 475.96 ng/μL, with an average value of 238.77 ± 89.18 ng/μL. Genotypic profiling was performed using RAPD-PCR analysis and the obtained electrophoretic profiles of the RAPD-PCR products were compared using hierarchical cluster analysis to eliminate identical bacterial isolates. Dendrogram analysis revealed that 30 strains were divided into 3 clusters, indicating that three genetically distinct strains were isolated from the sauerkraut brine. SDS-PAGE analysis revealed no dominant protein bands with a molecular weight of about 50 kDa, indicating that the strains isolated in this work most likely do not produce S-proteins.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:275051
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-11-14 13:36:27