The modern, bustling way of life and modernization process has led to numerous changes in the lives of children from rural and urban environments. Today's children are influenced by a number of modern technologies that are increasingly occupy them, leading to less time spent playing outdoor sports, physical activity, sports and socializing with friends. For this reason, a research was conducted to determine the differences in the motor skills of pre-school children attending a kindergarten from rural and urban environments. For the purpose of this study, a total of 79 children were measured, at an average age of 5.75, in two kindergartens in rural environment and two kindergartens in urban environment. Six motor abilities were measured: balance, coordination, repetitive power, explosive power, speed and flexibility. Throughout the work, basic theoretical and methodological starting points are used for research purposes. The basic concepts and the individual researches in Croatia and in the world related to the subject of this research and based on the comparison were explained. The results indicate that there is a statistically significant difference between children from rural and urban environments. Children from urban environments are better in motor skills and there is a statistically significant difference in the results of tests for repetitive power, coordination and speed testing. Only in flexibility test, children from rural environment showed statistically better results. The results obtained may be due to the better financial ability of parents to ensure that children are engaged in certain sports as well as the proximity of the training venue. Rural children are not in great ability to deal with a particular sport or organized physical activity because of the lack of training in their vicinity. Therefore, it is for educators and parents to enable children to stay outdoors and play because it is well known that pre-school is the most favorable time for optimal growth and development of the child.