The Department of Zoology in the Croatian Natural History Museum (HPM) in Zagreb is a leading center for biodiversity research in Croatia, with a collection of over two million specimens. The historical development of the department can be traced back to the establishment of the National Museum in 1846, highlighting the contributions of curators and naturalists in gathering and preserving the collections. The paper describes methods of acquisition and preservation of museum items, as well as the importance of maintaining an optimal microclimate for museum collections. The paper addresses challenges such as the damage to the collection caused by the 2020. earthquake in Zagreb, and the significance of accurate documentation and inventory of items. Despite the lack of financial resources and specialized staff, the Department of Zoology actively works on the preservation and research of national fauna. The museum staff is preparing for the relocation of the collection as part of the opening of a new exhibit in the Upper Town, planned for September 2024. The work includes an interview with senior curator Vlatka Mičetić Stanković, who highlights the daily challenges and future plans for the museum, emphasizing the importance of preserving biodiversity and introducing museum activities to the general public.