Basil (Ocimum basilicum L.) is one of the most important and versatile aromatic and medicinal plant, while phosphorus is one of the most important and frequently limited nutrient for plant growth. Arbuscular mycorrhiza (AM) is present in more than 90 % of terrestrial plant species. In conditions of nutrient deficiency in the soil, AM can significantly increase the efficiency of nutrient uptake, and thus avoid the stress triggered by lack of nutrient in host plants. Aim of current study was to determine the influence of AM on phosphorus uptake, the frequency and intensity of root mycorrhization and morphological characteristics of roots among different basil cultivars. The experiment included four basil cultivars ('Genovese', 'Sweet Basil', 'Dark Opal' and variety purpurascens) which were grown under low phosphorus availability (1 μmol P L-1) for 60 days. The treatments were represented as root inoculation with AM mycorrhizal species Glomus intraradices and uninoculated plants as control. At the end of experiment, shoot phosphorus concentration, the intensity and frequency of root mycorrhization and morphological characteristics of root system were determined. AM inoculation increased phosphorus concentration in dry matter in all inoculated basil cultivars compared to uninoculated cultivars. The highest concentration of P2O5 in the dry matter of plant shoot system was recorded in variety purpurascens. Significant difference in intensity and frequency of mycorrhization where observed among basil cultivars, where strongest infection was determined in 'Dark Opal', with intensity of mycorrhization of 54.0 %, and mycorrhization frequency of 70,6 %; and the least infected was var. purpurascens, with intensity of mycorrhization of 17,0 %, and mycorrhization frequency of 36,6 %. Cultivars with the highest frequency and intensity of mycorrhization, 'Sweet Basil' and 'Dark Opal', had weakest developed root system.