Caper (Capparis orientalis Veill.) is perennial shrub that grows predominantly in the Mediterranean region. Capers play an important role in food production and have become highly valuable commercial product. Conventional method of caper reproduction by planting seeds is not desirable for two main reasons: low germination rate due to the dormancy of the seed and cross-pollination, which results in a high level of heterozygous seed. Cultivation of capers requires large quantity of seedlings and these quantities can be supplied by micropropagation. The aim of this study was to explore the influence of three sources of lights on rooting of capers in vitro and on success of acclimatization of micropropagated capers in ex vitro conditions. During experiments, uniformed sprouts of micropropagated capers of 1-1,5 cm in length have been used as explants and have been cultivated on an MS medium with addition of 0,6 mg/l meta-Topolin (mT). Three sources of artificial light have been used in experiments: fluorescent lamps; LED lights with red to blue ratio of 70% to 30%; LED broad spectrum light: 35 % of red: 21% of blue: 39% of green: 6% of far red light. The results of research indicate that LED broad spectrum light has a positive impact on the measured root characteristics: root length, surface and volume of roots as well as on density of stomata and concentration of photosynthetic pigments. The results also indicate that treatments do not result in significant differences in percentage of acclimatized plants.