The problem with metals in agricultural environments is enhanced by their potential for mobilization under specific physical and chemical soil conditions. Direct effect of this mobilization is faster translocation of metals into deeper soil layers, and thereby possible contamination of groundwater. The problem with metal mobility on sloping terrains is exacerbated by erosion processes making the assessment of metal mobility risk extremely demanding and complex. Increased mobility of metals in the soil is a frequent effect of fertilization. The aim of this study is to determine if there are differences in the sorption and desorption of copper in the adsorption complex between two selected types of vineyard soils. A laboratory experiment of sorption and desorption of copper was carried out on soil samples collected in vineyards at two location: experimental station Jazbina (0-15 and 75-100 cm) and experimental vineyard Baštica (0-25 and 75-100 cm). In these experiments copper (II) nitrate solution was used in various concentrations (25, 50, 100 and 200 mg/l) and all measurements were made in triplicate. Sorption isotherms were constructed for all soil horizons studied. The highest sorption and retention capacity was determined in pseudogley on slope, experimental station Jazbina in topsoil (0-15 cm), which was confirmed by the highest distribution coefficient (99,003 ml/g). Furthermore, the lowest sorption and retention capacity was determinated in rigosol, experimental vineyard Baštica, at a depth of 75 to 100 cm. Based on the results of the sorption experiment, copper sorption isotherms are constructed for both experimental sites, which fit very well with the Freundlich model of isotherms according to the high correlation coefficient (R2 = 0.9999). Based on the obtained results it can be concluded that copper is a relatively immobile and less bioavailable metal that is most accumulated in the topsoil.