Grapevine is a perennial crop that is propagated vegetatively and currently there exists several thousands of varieties in the world. The variety ‘Kleščec bijeli’ (Vitis vinifera L.) is grown on limited areas in only several locations, among others around Križevci. In the past, ‘Kleščec bijeli’ has been neglected and considered to be a synonym with the German variety ‘Knipperle’ which is also known as ‘Ortlieber’. It was recently discovered that the variety ‘Kleščec bijeli’ is a unique genotype and can be considered as a new Croatian autochthonous variety (Pejić et al., 2013.) and a project was initiated to revitalize the variety using sanitary and genetic selection of clonal candidates. After many years of vegetative propagation within the grape varieties spontaneous mutations may occur and due to the nature of propagation they remain fixed in the genome (Meneghetti et al., 2012.). Selecting the vines whose characteristics deviate from the average of the variety is the backbone of clonal selection, which combined with the selection of virus-free vines provides verified and varietal purity of planting material. The aim of this study is to analyze the intravarietal variability of the clonal candidates of ‘Kleščec bijeli’, which are free from economically important viruses, and were used as mother vines for a mother block of the variety. In this study young leaves of 20 virus-free clonal candidates of ‘Kleščec bijeli’ were used. DNA was isolated after freeze-drying using the PeqGold Plant kit (Peqlab GmbH, Germany). AFLP analysis was performed using 4 primer combinations (Vos et al. 1995.). After the PCR reaction, fragments were separated electrophoretically using an ABI 3130 genetic analyzer and the fragment sizes were determined using a GeneMapper 4.0 software package (AppliedBiosystems, USA). Resulting fragment sizes were used for the construction of a binary matrix which was used for the calculation of a "Simple Matching" similarity coefficient which was used to evaluate the intravarietal variability of ‘Kleščec bijeli’.