A fertilization experiment with burley tobacco has been set up at the University of Zagreb Probationary Faculty of Agronomy in Maksimir during 2018. The experiment was set up according to the random block method in four replicates. Standard agrotechnics for burley tobacco were implemented, except for the savings. Nitrogen is added to the feed in five different variants: 1. Control of 100 kg / ha of KAN, 2. 90 kg / ha of KAN + 10 kg / ha of microcapsules, 3. 80 kg / ha of KAN + 20 kg / ha of microcapsules, 4. 90 kg / ha of KAN + 10 kg / ha of microcapsules with chitosan, 5. 80 kg / ha of KAN + 20 kg / ha of microcapsules with chitosan. Planting was done manually on May 11, 2018 by. F1 hybrid BH 4. A sample of 10 plants during the growing season determined the number of leaves, the length and width of the 9th leaf, the height of the plant after topping, and the days to flowering on the entire plot. The leaves were harvested by hand in seven harvests and air-cured in the shade. After drying, the yield (kg / ha) was determined and the tobacco was classified into six classes. From chemical compounds, the content of nicotine in the dry middle leaves of tobacco was determined. On the basis of the statistical analysis of variance, significant differences were found in the shares of the first class, the width of the 9th leaf, the height of the plant after topping and in the days to flowering. The highest yield per hectare had a variant of feeding 90 kg / ha KAN + 10 kg / ha chitosan microcapsules (3119.71 kg / ha), while the highest stake of first class leaf, significantly higher than other variants, had control ( 10.87%).The longest 9th leaf was recorded at the variant of feeding 80 kg / ha KAN + 20 kg / ha microcapsules (58.28 cm), and significantlly the widest 9. leaf had a variant of feeding 80 kg / ha KAN + 20 kg / ha microcapsules with chitosan (35.43 cm).