Sulphur (S) deficiency in soils is an increasingly common problem in many parts of the world as a result of different soil management and agronomic measures, high S biomass uptake, but also reduced S emissions from atmosphere. Sulphur along with nitrogen is indispensable in protein synthesis and adequate stocks of S in the soil also contribute to optimal crop yield, so knowledge of the contribution of mineral nitrogen fertilization to the content of S in the soil is very important to maintain soil production role. The aim of this thesis topic was to determine the vertical variability of total sulphur in stagnosol due to increasing doses of nitrogen fertilization, depth, and soil pH and soil texture. The research included the analysis of soil samples collected in 2007 from a long term field experiment located in the central part of Croatia with four increasing doses of nitrogen fertilization in the range of 0-300 kg N/ha. Sulphur was determined by dry incineration in 128 soil samples. Increasing doses of mineral nitrogen fertilization significant increased the total sulphur content in the soil up to 100 cm depth. Particular variability with respect to nitrogen fertilization was found in the surface soil layer where the sulphur content ranged from 0.0151% on the control treatment to 0.0373% on the N300 treatment, which is proportional to the increase of 1.5 times. The vertical distribution of total sulphur up to two meters deep varied significantly and its content decreased with depth. Overall, depending on the depth and doses of nitrogen fertilization, the sulphur content varied from 0.0102% on the N100 treatment on 150-175 cm depth to 0.0373% on the N300 treatment in the surface soil layer (0-25 cm). A significant and strong positive correlation was found between total sulphur and the silt and clay, as well as a significant, strong and negative correlation between the soil pH and total sulphur. The results also indicate that 26.1 t/ha of total sulphur was stored up to 2 meter of depth on N300 treatment.