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master's thesis
Genotipizacija varaždinskog kupusa pomoću AFLP markera
Zagreb: University of Zagreb, Faculty of Agriculture, 2016. urn:nbn:hr:204:868959
Maretić, Renato
University of Zagreb
Faculty of Agriculture
Plant Breeding Genetics, Biometrics and Experimentation

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Maretić, R. (2016). Genotipizacija varaždinskog kupusa pomoću AFLP markera (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:868959

Maretić, Renato. "Genotipizacija varaždinskog kupusa pomoću AFLP markera." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:868959

Maretić, Renato. "Genotipizacija varaždinskog kupusa pomoću AFLP markera." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:868959

Maretić, R. (2016). 'Genotipizacija varaždinskog kupusa pomoću AFLP markera', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 03 October 2024, https://urn.nsk.hr/urn:nbn:hr:204:868959

Maretić R. Genotipizacija varaždinskog kupusa pomoću AFLP markera [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2016 [cited 2024 October 03] Available at: https://urn.nsk.hr/urn:nbn:hr:204:868959

R. Maretić, "Genotipizacija varaždinskog kupusa pomoću AFLP markera", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:204:868959

Metadata
TitleGenotipizacija varaždinskog kupusa pomoću AFLP markera
Title (english)Genotyping of 'Varaždinski' cabbage using AFLP markers
AuthorRenato Maretić
MentorSnježana Bolarić (mentor)
Committee memberSnježana Bolarić (predsjednik povjerenstva)
Committee memberIvanka Žutić (član povjerenstva)
Committee memberSnježana Kereša (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Plant Breeding Genetics, Biometrics and Experimentation)
Zagreb
Defense date and country2016-06-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Tradicionalni kultivar 'Varaždinski kupus' od velikog je gospodarskog i kulturnog značaja. Njegovim sjemenarstvom bavi se još samo nekoliko obiteljskih poljoprivrednih gospodarstava, uglavnom za vlastite potrebe. Komercijalno dostupno sjeme je stranog porijekla i upitne srodnosti. Srodnost je proučavana pomoću AFLP molekularnih markera za čije proučavanje su bile korištene četiri polimorfne početnice. Proučavano je pet populacija: Varaždinski kupus iz Nacionalne banke biljnih gena (Var), populacije Varaždinskog kupusa obiteljskih gospodarstava OPG Hladika (Hld), OPG Plantić (Pln), OPG Cafuk (Caf) i komercijalno dostupno sjeme talijanske sjemenske kuće „Vita Bella“ (Vit). Detektirano je ukupno 203 markera od čega ih je 174 (86%) bilo polimorfnih. Analizom molekularne varijance utvrđena je signifikantna razlika između proučavanih populacija (ΦST:0.21282; p<0.001). Najveći udio fenotipske varijance opada na variranje unutar populacija (78.72%). Analizom međupopulacijskih udaljenosti utvrđena je signifikantna razlika između svih ispitivanih populacija (p<0,05%). Najveća udaljenost utvrđena je između populacija Vit i Pln (0,23), a najmanja udaljenost utvrđena je između populacija Caf i Pln (0,09). Analizom glavnih koordinata utvrđeno je grupiranje individua proučavanih populacija Varaždinskog kupusa u dvije glavne skupine. Jednu skupinu čine individue populacija Vit, a drugu populacije Var, Hld, Pln i Caf. Rezultati istražvanja važan su doprinos u poznavanju genetske varijabilnosti populacija Varaždinskog kupusa koji se mogu iskoristiti za zasnivanje organiziranog sjemenarstva Varaždinskog kupusa u Republici Hrvatskoj.
Abstract (english)
Traditional landrace of ‘Varaţdinski’ cabbage is of a great cultural and economical meaning. There are only few family farms that are producing its seed, mainly for personal use. Commercially available seed is produced in foreign countries and has questionable origin. Genetic similarity is analyzed by AFLP molecular markers with four polymorphic primers. Five populations have been studied: ‘Varaždinski’ cabbage from National bank of plant genes (Var), populations of ‘Varaždinski’ cabbage from family farms OPG Hladika (Hld), OPG Plantić (Pln), OPG Cafuk (Caf) and commercially available seed from Italian seed production company ‘Vita Bella’. It has been detected in total of 203 markers and 174 (86%) were polymorphic. By analysis of molecular variance we have established significant difference between studied populations (ΦST:0.21282; p<0.001). Most of the phenotypic variance belongs to variance between populations (78.72%). Analysis of distance between populations determined significant differences among analyzed populations (p<0,05%). Biggest distance was found between populations Vit and Pln (0,23), and the smallest was between populations Caf and Pln (0,09). Principal Coordinate Analysis defined clear vertical separation of two main groups. Population Vit forms first group and populations Var, Hld, Pln and Caf form the other group. The results of this research make a great contribution for gene variability of ‘Varaždinski’ cabbage and can be used for creating commercial seed production of ‘Varaždinski’ cabbage in Republic of Croatia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:868959
Study programmeTitle: Plant Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-08-29 12:13:36