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master's thesis
Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda
Zagreb: University of Zagreb, Faculty of Agriculture, 2021. urn:nbn:hr:204:876139
Parać, Marija
University of Zagreb
Faculty of Agriculture
Microbiology

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Parać, M. (2021). Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:876139

Parać, Marija. "Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:876139

Parać, Marija. "Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:876139

Parać, M. (2021). 'Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:204:876139

Parać M. Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2021 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:204:876139

M. Parać, "Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:204:876139

Metadata
TitleOtpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda
Title (english)Resistance to third generation cephalosporins in enterobacteria from wastewater
AuthorMarija Parać
MentorMirna Mrkonjić Fuka (mentor)
Committee memberMirna Mrkonjić Fuka (predsjednik povjerenstva)
Committee memberMarko Vinceković (član povjerenstva)
Committee memberVilim Filipović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Microbiology)
Zagreb
Defense date and country2021-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Cefalosporini treće generacije spadaju među najčešće korištene antibiotike za tretiranje raznih infekcija kojima su uzročnici enterobakterije. Njihovo masovno korištenje potaknulo je razvoj otpornosti kod enterobakterija koje se sve češće pronalaze u otpadnim vodama. Cilj ovoga rada bio je odrediti prisutnost gena za otpornost na cefalosporine treće generacije, poglavito gena koji kodiraju β-laktamaze proširenog spektra djelovanja (ESBL) u enterobakterija iz zagrebačkih komunalnih i bolničkih otpadnih voda te utvrditi specifične genske varijante. U analiziranih 104 izolata detektirana je prisutnost različitih ESBL gena (blaCTX-M, blaTEM, blaSHV, blaGES) primjenom lančane reakcije polimerazom (PCR) i agarozne gel elektroforeze. Sekvenciranjem umnoženih gena utvrđena je prisutnost 12 genskih varijanti. Većina izolata (85,58%) posjedovala je 2 ili više ESBL gena, dok je manji udio izolata (16,35%) posjedovao 3 ili više ESBL gena uz pojavu višestruko otpornog fenotipa. Rezultati ovog istraživanja ukazuju da su bolničke i obrađene komunalne otpadne vode rezervoari ESBL-producirajućih enterobakterija, često višestruko otpornih, što predstavlja rizik za daljnje širenje tih bakterija u okoliš i potencijalni prijenos na ljude.
Abstract (english)
Third-generation cephalosporins are among the most commonly used antibiotics to treat various infections caused by enterobacteria. Their widespread use has induced the development of resistance in enterobacteria, which are increasingly found in wastewater. The aim of this study was to determine the presence of third generation cephalosporin resistance genes, especially genes encoding extended spectrum β-lactamases (ESBL) in enterobacteria from Zagreb municipal and hospital wastewater and to identify specific gene variants. In the analyzed 104 isolates, the presence of various ESBL genes (blaCTX-M, blaTEM, blaSHV, blaGES) was detected using polymerase chain reaction (PCR) and agarose gel electrophoresis. Sequencing of amplified genes revealed the presence of 12 gene variants. Most isolates (85.58%) possessed 2 or more ESBL genes, while a smaller proportion of isolates (16.35%) possessed 3 or more ESBL genes with the appearance of a multidrug-resistant phenotype. The results of this study indicate that hospital and treated municipal wastewater are reservoirs of ESBL-producing enterobacteria, often multi-resistant, posing a risk for further spread of these bacteria to the environment and potential transmission to humans.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:876139
Study programmeTitle: Agroecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to authenticated users
Terms of useLicence In copyright icon
Created on2021-11-24 11:45:31