Entomopathogenic nematodes are soil nematodes that feed on bacteria. Together they can infect and kill several host insects. The use of entomopathogenic nematodes in plant protection poses a lower risk to non-target organisms compared to chemical insecticides. Therefore, they are environmentally friendly and are ideal for biological pest control. The most important entomopathogenic nematodes belong to the families Heterorhabditidae and Steinernematidae. Entomopathogenic nematodes can be produced through in vivo or in vitro processes and they can be very easily released into nature where they naturally renew themselves. So far, species of the genus Steinernema have been identified in the Republic of Croatia in the localities of Arduševac, Karane and Đeletovci. It is assumed that they are present in other areas in the Republic of Croatia, so the aim of this investigation was to determine the presence of entomopathogenic nematodes on agricultural land in continental Croatia. For the purposes of this work, soil samples were collected in nine localities of continental Croatia. The isolation procedure of entomopathogenic nematodes was carried out according to the methods of "Galleria bait" and "White trap". A total of 45 soil samples were analyzed, and entomopathogenic nematodes of the genus Steinernema were found only in one sample at the location Luka, which is 2.22 % of the total analyzed soil samples.