| Abstract | Proizvodnja silaža predstavlja konzerviranje zelene mase pri čemu je proizvodnja mliječne kiseline i hlapivih masnih kiselina (HMK) koja se događa tijekom siliranja najvažniji faktor koji utječe na konzerviranje. Bakterije mliječne kiseline često se dodaju prilikom siliranja u obliku inokulanta kako bi se povećala proizvodnja kiselina. Cilj ovog rada je istražiti utjecaj dodatka inokulanta na proizvodnju mliječne kiseline, HMK i etanola u silaži talijanskog ljulja u prvih 10 dana siliranja. U uzorcima silaža uzorkovanih 1, 3, 5 i 10 dan i u zelenoj masi odredila se koncentracija promatranih parametara HPLC metodom. U prvih 10 dana siliranja, u silaži talijanskog ljulja došlo je do povećanja mliječne kiseline, HMK i etanola (P<0,001). Pri tome je dodatak inokulanta povećao proizvodnju mliječne kiseline (97,77 vs 53,59 g/kg ST; P<0,001) i izomaslačne kiseline (0,92 vs 0,51 g/kg ST; P<0,01), smanjio proizvodnju octene kiseline (3.77 vs 10,40 g/kg ST; P<0,001) i propionske kiseline (0,22 vs 1,02 g/kg ST; P<0,001), dok na proizvodnju etanola nije značajnije utjecao (6,07 vs 6,63 g/kg ST; P>0,05). Navedeni rezultati potvrda su intenzivne proizvodnje mliječne kiseline i HMK u prvim danima siliranja silaža te da dodatak inokulanta značajno utječe na profil sintetiziranih kiselina. |
| Abstract (english) | Silage production is the conservation of plant green mass in which lactic acid and volatile fatty acids (VFA) are produced during ensiling. Acid production is the most important factor influencing conservation. Lactic acid bacteria are often added during ensiling in the form of inoculants to increase acid production. The aim of this study was to investigate the effect of inoculant application on lactic acid, VFA and ethanol production in Italian ryegrass silage in the first 10 days of ensiling. The concentration of all parameters was determined by HPLC in silage samples sampled on 1, 3, 5 and 10 days and in green mass. In the first 10 days of ensiling, there was an increase in lactic acid, VFA and ethanol in all silage samples (P<0.001). The application of inoculant increased the production of lactic acid (97.77 vs 53.59 g/kg ST; P<0.001) and isobutyric acid (0.92 vs 0.51 g/kg ST; P<0.01), decreased production of acetic acid (3.77 vs 10.40 g/kg ST; P<0.001) and propionic acid (0.22 vs 1.02 g/kg ST, P<0.001) while ethanol production was not significantly affected (6.07 vs 6.63 g/kg ST; P>0.05). The results are confirmation of intensive lactic acid and VFA production in the first days of Italian ryegrass ensiling. Obtained results confirmed that the profile of synthesized acids is significantly affected with inoculant application. |
