Stinging nettle (Urtica dioica L.) is a perennial wild species, which harvested before flowering, is used as a nutritionally valuable food. Due to numerous advantages compared to wild plant material, stinging nettle is increasingly cultivated. Traditional open filed cultivation is becoming more challenging over the years due to climate changes and market demand. Modern hydroponic techniques without soil, such as floating hydroponics, are used as an alternative cultivation method. The advantages of this cultivation method are numerous, such as the possibility of year-round cultivation, control of abiotic factors, and higher yield. In this master thesis, the influence of two nutrient solutions of different composition (Jensen and Cooper) on the yield and mineral composition of nettle grown in floating hydroponics under repeated harvest was studied. For morphological (leaf properties) and chemical properties (dry matter, nitrate content, phosphorus, magnesium), the solution prepared according to Jensen's recipe proved to be better than the solution prepared according to Cooper with a higher salt concentration. Regardless of solution composition, higher plant height and yield were measured after the second harvest, as well as higher content of dry matter, nitrate, potassium, magnesium, and iron. The combination of the Jensen solution and the second harvest resulted in with higher values of most traits and can be recommended for hydroponic nettle cultivation to obtain herbs with better morphological properties and a high nutritive final product.