Diseases and pests are a great concern for the human food industry. The appearance of diseases and pests results in large economic losses of manufacturers. When it comes to storing agricultural products, currently diseases and pests are treated by cleaning or treating with fungicides and insecticides. In order to avoid the use of pesticides, in society is currently necessary to find some new eco-friendly methods. One of the potential methods is the use of ozone, which has been studied in this work on strawberries. The strawberries have a very thin skin, and it is very problematic to store them because of their easy perishability. Main goal of this study was to determine the effectiveness of ozone in combating diseases and pests on strawberries. In the experiments carried out, different durations of ozone exposure and different number of days of treatments were examined. The results did not show the adverse effects of ozone on Botrytis assuming that this was due to adverse conditions during the conduct of the experiment. It is necessary for new research to be carried out with different concentrations of ozone and in controlled storage conditions.