The purpose of the research was to determine the effect of Actiwave, Humistar and Phylgreen biostimulants on the mineral composition and yield of marketable and share of nonmarketable tomato fruits grown in hydroponic system. A two-factorial experiment was conducted in 2019 at the experimental field of the Department of Vegetable Crops, Faculty of Agriculture. It was set up according to the random block design in three replications. In the experiment, two early hybrid tomato cultivars were tested: 'Alamina' and 'Vasanta' in four treatments: control (K), Actiwave (A), Humistar (H) and Phylgreen (P). In relation to the control, the positive impact of the biostimulants on the marketable fruit yield was achieved only in the 'Alamina' cultivar. The highest yield of marketable fruits of 13.83 kg/plant was achieved in plants treated with Phylgreen, followed by Humistar, while the control (10.75 kg/plant) and Actiwave (10.62 kg/plant) achieved a significantly lower yield. All three biostimulants, in all harvest times, in both cultivars, had a positive impact on the accumulation of most macro and microelements in the tomato fruit, except for Mg and Cu. A significantly higher content of N, P and Mg, and a lower content of Ca was found in the 'Vasanta' cultivar compared to 'Alamina' cultivar, while the potassium content was significantly higher only in the second sampling. The situation is similar with microelements, a higher content of Zn, Mn and Cu was determined in all samplings, and Fe in the first sampling in the 'Vasanta' cultivar compared to 'Alamina' cultivar.