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master's thesis
Djelovanje apitoksina na višestruko otporne bakterije
Zagreb: University of Zagreb, Faculty of Agriculture, 2022. urn:nbn:hr:204:990818
Čukac, Nikolina
University of Zagreb
Faculty of Agriculture
Department of Microbiology

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Čukac, N. (2022). Djelovanje apitoksina na višestruko otporne bakterije (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:990818

Čukac, Nikolina. "Djelovanje apitoksina na višestruko otporne bakterije." Master's thesis, University of Zagreb, Faculty of Agriculture, 2022. https://urn.nsk.hr/urn:nbn:hr:204:990818

Čukac, Nikolina. "Djelovanje apitoksina na višestruko otporne bakterije." Master's thesis, University of Zagreb, Faculty of Agriculture, 2022. https://urn.nsk.hr/urn:nbn:hr:204:990818

Čukac, N. (2022). 'Djelovanje apitoksina na višestruko otporne bakterije', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:204:990818

Čukac N. Djelovanje apitoksina na višestruko otporne bakterije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2022 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:204:990818

N. Čukac, "Djelovanje apitoksina na višestruko otporne bakterije", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:204:990818

Metadata
TitleDjelovanje apitoksina na višestruko otporne bakterije
Title (english)The effect of apitoxins on multidrug-resistant bacteria
AuthorNikolina Čukac
MentorMirna Mrkonjić Fuka (mentor)
Committee memberMirna Mrkonjić Fuka (predsjednik povjerenstva)
Committee memberLidija Svečnjak (član povjerenstva)
Committee memberDragan Bubalo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Department of Microbiology)
Zagreb
Defense date and country2022-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Nekritička uporaba antibiotika uzrokovala je globalnu pojavu rezistencije bakterija na konvencionalne antibiotike. Stoga je potrebno pronaći alternativna rješenja za sprječavanje njihovog rasta. U ovom je radu ispitan inhibitorni utjecaj uzoraka apitoksina (n=3) na sojeve višestruko rezistentnih bakterija Acinetobacter baumanii, Klebsiella pneumoniae, Enterococcus faecium i Staphylococcus aureus (n=20). Zone inhibicije rasta, određene metodom difuzije agara u bunaru, pokazuju da su Gram pozitivne bakterije osjetljivije (11,9 mm ± 0,7 mm) na djelovanje apitoksina od Gram negativnih bakterija (10,8 mm ± 4,1 mm; ANOVA, Bonferroni test: p = 0,03). Minimalna inhibitorna (MIK) i minimalna baktericidna (MBK) koncentracija uzoraka apitoksina iznosile su između 0,13 i 31,25 μg/ml. Utvrđeno je kako su uzorci apitoksina djelotvorni u inhibiciji rasta svih ispitivanih vrsta, ali pri različitim koncentracijama. Rezultati su pokazali i kako nema značajne razlike u inhibiciji različitih sojeva iste vrste, kao ni između djelotvornosti različitih uzoraka apitoksina.
Abstract (english)
The uncritical use of antibiotics has caused the global emergence of bacterial resistance to conventional antibiotics. Therefore, it is necessary to find alternative solutions to prevent their growth. In this work, the inhibitory effect of apitoxin samples (n=3) on strains of multi-resistant bacteria Acinetobacter baumanii, Klebsiella pneumoniae, Enterococcus faecium and Staphylococcus aureus (n=20) was tested. Zones of growth inhibition, determined by the agar diffusion method in the well, show that Gram-positive bacteria are more sensitive (11.9 mm ± 0.7 mm) to the action of apitoxin than Gram-negative bacteria (10.8 mm ± 4.1 mm; ANOVA, Bonferroni test: p = 0.03). The minimum inhibitory (MIC) and minimum bactericidal (MBK) concentrations of apitoxin samples were between 0.13 and 31.25 μg/ml. It was found that apitoxin samples are effective in inhibiting the growth of all tested species, but at different concentrations. The results showed that there is no significant difference in inhibition between different strains of the same species, as well as between the effectiveness of different samples of apitoxin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:990818
Study programmeTitle: Agroecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-10-13
Terms of useLicence In copyright icon
Created on2022-10-13 10:36:01