| Abstract | Vinova loza je jedna od najznačajnijih poljoprivrednih kultura u Republici Hrvatskoj. Vinovu lozu može zaraziti velik broj virusa, a prema literaturnim podacima do danas je u lozi otkriveno 86 različitih virusa. Nisu svi virusi ekonomski značajni, no oni koji jesu su: virus mozaika gušarke (ArMV), virus lepezastog lista vinove loze (GFLV), uvijenosti lista vinove loze pridruženi virusi 1, 2 i 3 (GLRaV-1, GLRaV-2, GLRaV-3), virus pjegavosti lista vinove loze (GFkV), A-virus vinove loze (GVA) te B-virus vinove loze (GVB). Cilj ovog istraživanja bio je utvrditi prisutnost i učestalost pojave ekonomski značajnih virusa ArMV, GFLV, GLRaV-1 te GLRaV-3 kod autohtone sorte Babić u njenim glavnim uzgojnim područjima. Testirano je ukupno 232 uzorka prikupljenih sa 10 različitih lokacija. Za testiranje je korištena imunoenzimska ELISA metoda. Potvrđena je prisutnost svih ispitivanih virusa. Najzastupljeniji virus je bio GLRaV-3 (97,84%), a zatim GFLV (31,47%) te GLRaV-1 (5,17%). Utvrđena prisutnost ArMV od 3,02% je upitna zbog niske koncentracije virusa. Utvrđene su i mješovite infekcije od kojih je najčešća bila kombinacija GFLV + GLRaV-3 (29,31%). Od svih testiranih uzoraka, kod samo 4 (1,72%) nije utvrđena prisutnost virusa obuhvaćenih istraživanjem. |
| Abstract (english) | The grapevine is one of the most important agricultural crops in the Republic of Croatia. Grapevine can be infected by a large number of known viruses, and according to literature 86 different grapevine viruses have been discovered. Not all viruses are economically significant, but those considered as important are: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus – 1, 2 and 3 (GLRaV-1,GLRaV-2, GLRaV-3), grapevine fleck virus (GFkV), Grapevine virus A (GVA) and B (GVB). The aim of this study was to determine the presence and frequency of the economically important viruses ArMV, GFLV, GLRaV-1 and GLRaV-3 in the autochthonous variety Babić collected from its main growing areas. In total 232 samples were collected from 10 different locations. Samples were tested using the immunoenzymatic ELISA method. The presence of all viruses included in survey was confirmed. The most common was GLRaV-3 (97,84%), followed by GFLV (31,47%) and GLRaV-1 (5,17%). Determined ArMV frequency of 3,02% is questionable due to low virus concentration. Mixed infections have also been identified and the most common combination was GFLV + GLRaV-3 (29,31%). Of all the tested samples, only in 4 (1,72%) presence of studied viruses was was not confirmed. |
