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master's thesis
Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)
Zagreb: University of Zagreb, Faculty of Agriculture, 2022. urn:nbn:hr:204:616166
Jembrek, Davor
University of Zagreb
Faculty of Agriculture
Department of Plant Breeding Genetics, Biometrics and Experimentation

Institutional repository: Repository Faculty of Agriculture University of Zagreb

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Jembrek, D. (2022). Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:616166

Jembrek, Davor. "Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)." Master's thesis, University of Zagreb, Faculty of Agriculture, 2022. https://urn.nsk.hr/urn:nbn:hr:204:616166

Jembrek, Davor. "Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)." Master's thesis, University of Zagreb, Faculty of Agriculture, 2022. https://urn.nsk.hr/urn:nbn:hr:204:616166

Jembrek, D. (2022). 'Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 26 June 2024, https://urn.nsk.hr/urn:nbn:hr:204:616166

Jembrek D. Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2022 [cited 2024 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:204:616166

D. Jembrek, "Utjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:204:616166

Metadata
TitleUtjecaj citokinina na mikropropagaciju, morfologiju i razinu stresa kod kapara (Capparis orientalis Veill.)
Title (english)The effect of cytokinins on in vitro multiplication, morphology and oxidative stress of caper (Capparis orientalis Veill.) shoots.
AuthorDavor Jembrek
MentorAnita Bošnjak Mihovilović (mentor)
Committee memberAnita Bošnjak Mihovilović (predsjednik povjerenstva)
Committee memberSnježana Kereša (član povjerenstva)
Committee memberIvanka Habuš Jerčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Department of Plant Breeding Genetics, Biometrics and Experimentation)
Zagreb
Defense date and country2022-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Biljka kapara zbog niske klijavosti i visokog stupnja heterozigotnosti u sjemenu neprikladna je za razmnožavanje konvencionalnim metodama. Kao jedno od rješenja koje bi pospješilo i ubrzalo razmnožavanje ove biljne vrste je mikropropagacija. Najčešće korišteni citokinin u svrhu mikropropagacije kapara (C. orientalis Veill.) je 6-benziloaminopurin (BAP). Metatopolin (mT) se smatra alternativom BAP-u jer osigurava visoke stope umnožavanja uz manje negativnih posljedica. Upotreba citokinina koja je nužna za multiplikaciju in vitro može dovesti do stresnih uvjeta i povećane proizvodnje reaktivnih kisikovih oblika (ROS) koji ograničavaju mikropropagaciju jer dovode do morfofizioloških promjena i aktivacije enzima stresa. Cilj rada je bio odrediti učinak vrste i doze citokinina na stopu multiplikacije, lisnu površinu i razinu aktivnosti enzima stresa i ukupnih proteina u izdancima kapara u in vitro uvjetima.
Abstract (english)
Due to low germination and high degree of heterozygosity in the seeds, the caper plant is not suitable for propagation by conventional methods. Micropropagation is one of the solutions that would promote and speed up the reproduction of this plant species.The most used cytokinin for the purpose of micropropagation of caper (C. orientalis Veill.) is 6-benzylaminopurine (BAP). Metatopolin (mT) is considered as an alternative to BAP because it provides high multiplication rates with fewer negative consequences. The use of cytokinin, which is necessary for multiplication in vitro, can lead to stressful conditions and increased production of reactive oxygen species (ROS) that limit micropropagation. They lead to morphophysiological changes and activation of stress enzymes. The aim of the work was to determine the effect of the type and dose of cytokinin on the multiplication rate, leaf area and level of stress enzyme and protein activity in caper shoots in vitro.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:616166
Study programmeTitle: Plant Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-02 09:26:10