Colorado potato beetle (Leptinotarsa decemlineata Say) (CPB) is the most important potato pest. Due to its rapid adaptation and development of resistance, it creates major problems in potato pest protection. So far, 300 cases of CPB resistance on 56 insecticides have been reported worldwide. The resistance of CPB in Croatia has been proved on chlorinated hydrocarbons, organophosphorus insecticides, carbamates and pyrethroids. The aim of the study was to determine the susceptibility of CPB larvae to permitted insecticides in the Republic of Croatia (chlorpyrifos, cypermethrin, thiacloprid, chlorantraniliprole, metaflumizone, spinosad and lufenuron) on ten locations from six counties in continental Croatia. In 2018, laboratory tests were conducted according to the IRAC test method 007, by inoculating potato leaves into various groups of insecticides. Populations were classified into 5 categories based on efficacy of the recommended dose 48 hours after the treatment for fast acting insecticides and 72 hours after insecticide treatment for slow acting insecticides. All investigated Colorado potato beetle populations were resistant to cypermethrin, chlorantraniliprol and lufenurom. Half of the investigated populations was resistant to thiacloprid and metaflumizone. Chlorpyriphos showed efficacy only in one population, while other populations were resistant. All populations of Colorado potato beetle were susceptible to spinosad.