Viticulture in Croatia encompasses the cultivation of a large number of grape varieties and is based on the cultivation of autochthonous varieties. A problem that is becoming more and more pronounced is their high infection with vine viruses. The research was carried out on 10 autochthonous grapevine varieties sampled from the National Collection of autochthonous varieties, which were previously found to be infected with one or both of two new grapevine viruses, grapevine virus G (GVG) and grapevine badnavirus 1 (GBV-1). Apical meristem culture was used as a method of recovery from the virus, during which apical meristems of 1 mm size were isolated. Statistical analysis revealed a significant difference between the four selected varieties that have two repetitions (ˈOskorušicaˈ, ˈTanetova lozaˈ, ˈSilbijanacˈ and ˈPetovkaˈ) with regard to the % of grown individuals after 2 weeks, while no significant difference was found in individuals regenerated after 4 weeks. Sanitary status significantly influenced growth and regeneration in tissue culture, that is, individuals infected with GBV-1 achieved 77.8% regeneration, those infected with GVG 78.9% regeneration, respectively sanitary status significantly affects the % of explants grown after 2 weeks but not on the % of regenerated individuals after 4 weeks of introduction into tissue culture. By measuring the height after 5 weeks, a significant difference was found between the cultivars for the two height categories. After reaching height, a smaller percentage of plants were successfully acclimatized (3.1 %), and a very low percentage of plants achieved successful virus elimination from plant tissue by this apical meristem method (0.9 %).