The Velvetleaf is an aggressive weed of fallow crops (maize, soybeans, sunflower, sugar beet). With its competitive abilities, it affects the cultured plant, takes away the elements necessary for life and thus reduces its yield. Its suppression requires knowledge and expertise, and then large financial resources, thus directly affecting the economic profit of the agricultural economy. Biological control of the Velvetleaf using insects would greatly reduce the costs of its control and reduce the harmful impact of herbicides on the environment, beneficial organisms and humans. In the biological control of weeds, organisms are used that have been proven to feed exclusively on the target species, without harmful effects and danger to cultivated plants. The aim of this work was to determine the entomofauna of the Velvetleaf species and to determine their effect and potential for use in the biological control of the Velvetleaf. The research was conducted in the Požega-Slavonia County, at six locations (Bučje, Jakšić, Vidovci, Velika, Pleternica and Kutjevo) in the period from May to August 2023. The entomofauna was determined by visual inspections in the tillage crops (maize, soybean, sunflower and sugar beet), in which the Velvetleaf appeared. During the examination of the plants, the occurrence of damage to the plant organs caused by the feeding of the observed insects was monitored. Accidental species that have been found on Velvetleaf plants are Eurydema ventralis Kolenati, Coreus marginatus L., Phyllotreta sp. and Agriotes sp. for which nutrition has not been determined. Of the harmful species, aphids, Ostrinia nubilalis Hb., Liorhyssus hyalinus F., Carcharodus alceae Esp. and Heliothis armigera Hb.