The aim of this study was to determine the impact of land use on selected properties of Humofluvisol. The study was conducted in year 2023 on experiment station of the Faculty of Agriculture in Zagreb on 4 locations, under different land uses of: arable land, meadow, vegetable garden and orchard. Three average top-soil samples (0-30 cm) were taken from each location. The particle size distribution (texture), pH, content and character of humus, carbonates, K2O and P2O5, were determined in the soil samples. A silty loam texture was determined in all soil samples. A significantly lower pH values were determined in the soil of orchard in comparison to the other study locations. A significantly higher humus content was determined in the meadow (4.40 %) compared to the soil used in agricultural production (2,47-3,11%). The orchard soil was non-calcareous, and the other soils were slightly calcareous (0,64-1,95%). Soil in arable land and vegetable production had an average higher concentration of physiologically active phosphorus (18,75 and 22,08 mg/100 g of soil, respectively) in comparison to the soil from the meadow (9,36 mg/100 g of soil). Soil from the meadow had a significantly lower concentration of P2O5 (10,48 mg/100 g of soil) in comparison to the soil from the arable land and the vegetable garden. The average concentration of K2O in the orchard soil (22,5 mg/100 g of soil) was significantly higher in comparison to the soil under natural vegetation (14,6 mg/100 g of soil).