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master's thesis
Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela
Zagreb: University of Zagreb, Faculty of Agriculture, 2023. urn:nbn:hr:204:234675
Galešić, Marija Andrijana
University of Zagreb
Faculty of Agriculture
Agricultural Zoology

Institutional repository: Repository Faculty of Agriculture University of Zagreb

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Galešić, M. A. (2023). Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:234675

Galešić, Marija Andrijana. "Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:234675

Galešić, Marija Andrijana. "Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:234675

Galešić, M. A. (2023). 'Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:204:234675

Galešić MA. Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2023 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:204:234675

M. A. Galešić, "Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:204:234675

Metadata
TitlePrimjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela
Title (english)Applied molecular analysis regarding microbiotic fecal residue of wild bee species
AuthorMarija Andrijana Galešić
MentorDarija Lemić (mentor)
Committee memberDarija Lemić (predsjednik povjerenstva)
Committee memberLidija Svečnjak (član povjerenstva)
Committee memberMirna Mrkonjić Fuka (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Agricultural Zoology)
Zagreb
Defense date and country2023-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Pčele kao glavni biljni oprašivači imaju ključnu ulogu u održavanju ravnoteže cjelokupnog ekosustava. Solitarne pčele su nerijetko s u kontaktu sa medonosnim pčelama kao zadružnim kukcima te mogu međusobno utjecati na sastav crijevne mikrobiote koja utječe na zdravlje jedinki, ali i cijele zajednice. Provedbom DNA metabarkodiranja uzoraka fecesa triju vrsta solitarnih pčela (Hoplitis adunca, Osmia bicornis, Megachile sculpturalis) utvrđene su prehrambene preferencije i prisutnost mikroorganizama u crijevnoj mikrobioti jedinki. Biljne preferencije prilikom ishrane ukazuju na biljke roda Echium sp., Prunus sp. i Ranunculus sp. Analizom bakteriološkog sastava crijevne mikrobiote utvrđena je prisutnost bakterije Melissococcus plutonius te prisutnost bakterija Bradyrhizobium sp. i Clostridium sp. Potrebna su daljnja istraživanja solitarnih pčela i njihovih prehrambenih i ekoloških navika kako bi se osigurao opstanak i poboljšanje broja njihovih populacija kao jednih od najvažnijih oprašivača u prirodi.
Abstract (english)
Bees, as primary plant pollinators, play a crucial role in maintaining the balance of the entire ecosystem. Solitary bees often come into contact with honeybees as social insects, and they can mutually influence the composition of the gut microbiota, which impacts the health of individuals as well as the entire community. By conducting DNA metabarcoding of fecal samples from three species of solitary bees (Hoplitis adunca, Osmia bicornis, Megachile sculpturalis), dietary preferences and the presence of microorganisms in the gut microbiota of individuals were determined. Plant preferences during feeding point to plants of the genus Echium sp., Prunus sp., and Ranunculus sp. Through analysis of the bacterial composition of the gut microbiota, the presence of the bacterium Melissococcus plutonius was established, along with the presence of bacteria Bradyrhizobium sp. and Clostridium sp. Further research into the dietary and ecological habits of solitary bees is necessary to ensure the survival and enhancement of their populations as one of the most important pollinators in nature.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:234675
Study programmeTitle: Agroecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-11-21 10:18:40