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master's thesis
Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza
Zagreb: University of Zagreb, Faculty of Agriculture, 2016. urn:nbn:hr:204:739674
Čuklić, Mario
University of Zagreb
Faculty of Agriculture
Animal Nutrition

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Čuklić, M. (2016). Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:739674

Čuklić, Mario. "Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:739674

Čuklić, Mario. "Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:739674

Čuklić, M. (2016). 'Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:204:739674

Čuklić M. Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2016 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:204:739674

M. Čuklić, "Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:204:739674

Metadata
TitleUtjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza
AuthorMario Čuklić
MentorDarko Grbeša (mentor)
Committee memberDarko Grbeša (predsjednik povjerenstva)
Committee memberGoran Kiš (član povjerenstva)
Committee memberZlatko Svečnjak (član povjerenstva)
Committee memberMarija Duvnjak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Animal Nutrition)
Zagreb
Defense date and country2016-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Primarni cilj siliranja je očuvanje hranjivih tvari i energije koja se nalazi u zelenoj masi kroz duži vremenski period. Konzervirajući učinak postiže se uz pomoć anaerobnih uvjeta i djelovanja bakterija mliječne kiseline. Najnovija istraživanja ukazuju da unatoč niskom pH mikrobna aktivnost ustraje i u stabilnoj fazi, što bi za posljedicu moglo imati i kontinuirane promjene u dušićnim frakcijama. Cilj istraživanja bio je odrediti utjecaj dodatka inokulanta i mliječne kiseline prilikom siliranja cijele biljke kukuruza na sadržaj sirovih proteina (SP) i amonijaka (NH3) tijekom perioda od 364 dana stajanja silaže. Osim navedenih frakcija promatran je i utjecaj na sadržaj suhe tvari. Silaže su silirane s dodatkom inokulanta Bio-Sil koji u sebi sadrži lifilizirane kulture Lactobacillus plantarum 8862 i 8866 te s dodatkom mliječne kiseline u linearnom rasponu od 1 % do 7 % ST mase silaže. Sirovi protein određen je Kjeldahlovom metodom, a sadržaj amonijskog dušika spektrofotometrijski korištenjem Nesslerova reagensa. Mliječna kiselina i inokulant nisu značajno utjecali na sadržaj suhe tvari i sirovih proteina. Tijekom 364 dana bilježen je konstantan porast amonijaka u tretiranim i kontrolnim silažama što je potvrda porasta proteolize i deaminacije u ispitivanim silažama. Najveći porast vrijednosti amonijaka zabilježen je u kontrolnim silažama (0−6,69 % SP), dok je proizvodnja amonijaka bila najmanja u silažama tretiranim mliječnom kiselinom (0−4,23 % SP). Kod silaža tretiranih inokulantom zabilježen je porast amonijaka od 0 do 6,29 % SP. Dodatak inokulanta smanjio je obujam proteolize i deaminacije što je najvjerojatnije uvjetovano dominacijom Lactobacillus plantaruma u takvim silažama. Dodatak mliječne kiseline umanjio je opću mikrobnu aktivnost što je rezultiralo smanjenjem ukupnih proteolitičkih aktivnosti i redukcijom sinteze amonijaka.
Abstract (english)
Ensiling is a method of preservation nutrients and energy located in forage for longer time periods. Preservative effect is achieved by establishing anaerobic conditions and by lactic acid bacteria activity in ensiled material. The latest studies indicate that despite low pH microbial activity persists in a stable phase. This activity can result in a continuous changes in nitrogen fractions. The subject of this study was to determine the influence of inoculant and lactic acid bacteria on crude protein (CP), ammonia (NH3) and dry matter (DM) content in whole plant corn silage during ensiling period of 364 days. Silages were ensiled with the addition of Bio-Sil inoculant which contains liofilisated cultures of Lactobacillus plantarum 8862 and 8866, and with the addition of lactic acid in the linear range from 1% to 7% DM silage mass. CP was determined by the Kjeldahl method and ammonium nitrogen by spectrophotometry using Nessler reagent. Lactic acid and inoculant did not significantly affect DM and CP. During 364 days a constant increase in ammonia in treated and control silages was recorded and is confirmation of an increase in proteolysis and deamination in tested silages. The highest ammonia level was recorded in control silages (0 to 6.69%of CP), while the production of ammonia was smallest in silages treated with lactic acid (0 to 4.23% of CP). In silages treated with inoculant ammonia content increased from 0 to 6.29% of CP. Inoculation reduced proteolysis and deamination extent possibly influenced by the domination of Lactobacillus plantarum in these silages. Addition of lactic acid reduced the overall microbial activity, resulting in a decrease of total proteolytic activity and reduction of ammonia synthesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:739674
Study programmeTitle: Animal Nutrition and Feed Science
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane (magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-11-30 13:50:25