Agrotourism, as a form of rural tourism has been increasing recently in Croatia. This form of tourism, which successfully combines agroculture and tourism services is becoming increasingly popular solution to the problem of finding additional sources of income on farms. As the clean and preserved environment is one of the main assumptions of agrotourism, question that occures is whether and how the owners of agrotourism farms in Croatia conducted its business in an environmentally responsible and sustainable manner. Therefore, the aim of this study was to examine the owners of farms on familiarity with the principles of environmentally responsible business, their opinions and attitudes of the same and examine the use of this method in business practices. The survey was conducted through online surveys of 60 agrotourism farms. The questionnaire was based on interviews that were conducted with 6 agrotourism farms in Croatia. The results of the online survey studies show that 55% of owners are familiar with the term "environmentally responsible", while 38% of respondents have heard of the term, but not sure what it means and what includes. Although there are differences in the manner and frequency of implementation of environmentally responsible practices on agrotouristic farms, all respondents considered the care for environmental protection important or very important for business of agritourism farm.