The grapevine leafminer is an invasive species of moth from the family Gracillariidae, originating from North America, and it was discovered in Europe in 1995. In Croatia, the species was identified in 2010, but its distribution and harmfulness are poorly researched. The larvae of the pest feed on the leaf parenchyma, creating long winding mines in the leaf, which reduces its photosynthetic area and causes the leaf to dry out prematurely. The aim of this study was to determine the presence of the grapevine leafminer in the vineyards of the Jazbina experimental station. Damage from the grapevine leafminer was determined by visual inspection of grapevines during the autumn of 2023 in a vineyard cultivating varieties resistant to downy mildew and powdery mildew. A total of 380 vines of different grape varieties were inspected, and damage was found on more than 20% of the inspected vines. On average, all investigated varieties had damage on two leaves per vine, with average damage of 1.5 cm² per leaf. The presence of the grapevine leafminer was confirmed for the first time at the Jazbina experimental station. Although significant damage to grapevine cultivation was not recorded, due to the invasive nature of the species and its rapid spread across Europe, it is recommended to regularly monitor its population.