In modern beekeeping the most attention is focused on increasing economic benefits, so the beekeeping production greatly depends on selection. In the selection of honey bees, the most important biological traits used as criteria in different selection programs are the speed of spring development, swarming tendency, aggressiveness, calmness on the comb, productivity and tolerance to diseases. Therefore, the aim of this study was to compare the dynamics of brood developement, number of bees on the comb, strength, behavior and honey production between honey bee colonies from three breeding lines of Carniolan bee (Apis mellifera carnica P.,1879). The study was conducted on a total of nine colonies, three colonies per three breeding lines (A, B and C). The colony strength, number of bees on the comb and development of brood were observed by the Liebefeld method every 15 days on each experimental colony and the behavior of the bees through aggressiveness, calmness on the comb and the swarming tendency was evaluated according to the standard criterion (Ruttner, 1972). Also, the honey production per colony was compared between the investigated lines. The results have revealed statistically significant differences between breeding lines A (18 734) and B (14 935), and lines B and C (19 312) in the average number of bees on the comb but were not determined for dynamics of brood development. A statistically significant difference between breeding lines B (39 202) and C (50 334) was established for the strength of the honey bee colonies. There was no statistically significant difference between the breeding lines for the aggressiveness and calmness on comb, while in the case of the swarming tendency it was observed between breeding lines A (2,90) and C (3,54), and B (2,58) and C. Considering the average honey yield the statistically significant difference between the breeding lines was not determined..