This thesis describes the procedure of conservation-restoration work which is done on the painting St. Anthony of Padua with the Infant Christ by an unknown author from the 18th /19th centuries. The work of art comes from a private property of family Reis and during the treatment/restoration it was stored in the Department for Conservation and Restoration of Works of Art at the Academy of Fine Arts in University of Zagreb. In consultation with the owner, Mrs. Jasmina Rais, it was decided to go on a complete renovation of the painting, but the great dilemma was with the subframe which was glued on the painting, so it was considered that maybe it was better to preserve the subframe and not remove it from the painting. In spite of the strainer frame's historical value, it was not safe to say that the interventions would give satisfying results, restoring it' s firmness and function so it was decied to make a new frame. Conservation-restoration documentation of the thesis consists of the written and photographed record, art history archive and research, diagnostic testing and conservation-restoration treatment. The section Conservation-restoration researches has all of the general informations about the painting, it's art history archive and research, iconographic analysis, the proposal of work, current situation of the painting and causes of deterioration. In the part of the thesis, Identification of the materials and analysis, described are the methods of the diagnostic researches with a goal to identify type of canvas, base, varnish and the compostion of the dirt at the back fo the canvas. Also, a stratigraphic cross-sectional analysis was performed on the sample of the painted layer and all the pigments. Interventions that were made on the painting and on the decorative frame were systematically photo-documented and described in stages of work, in chapter Conservation-restoration's work. The most demanding part of restoration was during the cleaning of the back of the canvas, but with the available literature, adequate funds and a professional mentorship satisfying results were obtained after cleaning. The works performed on the painting have been corrected with basic written, graphic and photographic documentation. At the end, painting will be returned to the owners.