prikaz prve stranice dokumenta UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)
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master's thesis
UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. urn:nbn:hr:181:455635
Pavičić, Martina
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

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Pavičić, M. (2013). UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:455635

Pavičić, Martina. "UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. https://urn.nsk.hr/urn:nbn:hr:181:455635

Pavičić, Martina. "UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. https://urn.nsk.hr/urn:nbn:hr:181:455635

Pavičić, M. (2013). 'UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:455635

Pavičić M. UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2013 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:181:455635

M. Pavičić, "UČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:181:455635

Metadata
TitleUČINAK TRANSGENA ZA KRIPTOGEIN NA AKTIVNOST FENILALANIN-AMONIJ-LIJAZE U KOSMATOM KORIJENJU UKRASNE KOPRIVE (Coleus blumei BENTH.)
Title (english)EFFECT OF ß-CRYPTOGEIN TRANSGENE ON PHENYLALANINE AMMONIA-LYASE ACTIVITY IN PAINTED NETTLE (Coleus blumei BENTH.) HAIRY ROOTS
AuthorMartina Pavičić
MentorElizabeta Has Schon (mentor)
Committee memberIvna Štolfa Čamagajevac (predsjednik povjerenstva)
Committee memberElizabeta Has Schon (član povjerenstva)
Committee memberLjiljana Krstin (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2013-10-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Akumulacija ružmarinske kiseline (RA) u biljkama se često javlja kao dio obrambenog odgovora biljke na stres i napad patogena. Prvi korak biosinteze RA, neoksidativnu deaminaciju L-fenilalanina pri čemu nastaje trans-cimetna kiselina, katalizira enzim fenilalanin-amonij-lijaza (PAL). U staničnoj kulturi ukrasne koprive pojačanoj sintezi RA prethodi pojačana aktivnost PAL. Cilj ovog rada da bio je istražiti utjecaj endogeno sintetiziranog oomicetalnog elicitora ß-kriptogeina na aktivnost enzima PAL i biosintezu ružmarinske kiseline. U tu svrhu uzgojeno je transgeno korijenje ukrasne koprive (Coleus blumei Benth.), u kojem se sintetički gen crypt nalazi pod kontrolom inducibilnog alkoholnog promotora, te tretirano etanolom kako bi se inducirala sinteza -kriptogeina. Ekspresija ß-kriptogeina povećala je aktivnost enzima PAL, ali je uzrokovala značajno smanjenje sadržaja ružmarinske kiseline u linijama transgenog korijenja.
Abstract (english)
The accumulation of rosmarinic acid (RA) in plants often occurs as part of the plant defense response to stress and pathogen attack. The first step of RA biosynthesis, non-oxidative deamination of L-phenylalanine to trans-cinnamic acid, is catalyzed by the phenylalanine ammonia-lyase (PAL) enzyme. In cell culture of painted nettle enhanced synthesis of RA precedes to increased PAL activity. The aim of this study was to investigate the effect of endogenously synthesized oomycetal elicitor -cryptogein on the PAL enzyme activity and rosmarinic acid biosynthesis. For this purpose transgenic roots of painted nettle (Coleus blumei Benth.) were grown, in which a synthetic gene crypt was under the control of alcohol inducible promoter and treated with ethanol to induce synthesis of -cryptogein. Expression of -cryptogein increased the activity of PAL enzyme but caused a significant decrease of rosmarinic acid content in the genetic lines of transgenic roots.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:455635
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-01-27 09:06:32