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master's thesis
Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. urn:nbn:hr:181:170025
Sabo, Nikolina
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

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Sabo, N. (2019). Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:170025

Sabo, Nikolina. "Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:170025

Sabo, Nikolina. "Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2019. https://urn.nsk.hr/urn:nbn:hr:181:170025

Sabo, N. (2019). 'Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:170025

Sabo N. Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2019 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:181:170025

N. Sabo, "Utjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:181:170025

Metadata
TitleUtjecaj selena na sadrţaj glutationa i antioksidacijsku aktivnost u stanicama alge Monoraphidium cf. contortum
Title (english)The effect of selenium on glutathione content and antioxidant activity in Monoraphidium cf. contortum
AuthorNikolina Sabo
MentorIvna Štolfa Čamagajevac (mentor)
MentorDubravka Špoljarić Maronić (komentor)
Committee memberRosemary Vuković (predsjednik povjerenstva)
Committee memberDubravka Špoljarić Maronić (član povjerenstva)
Committee memberTanja Žuna Pfeiffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2019-03-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Selen (Se) je element u tragovima koji se u prirodi pojavljuje u brojnim organskim i anorganskim oblicima. Dosadašnja istraţivanja pokazala su dvojak učinak Se na ţivi svijet – djeluje kao esencijalan mikronutrijent, ali i kao toksični element, u ovisnosti o koncentraciji, kemijskom obliku te drugim parametrima okoliša. Potencijalna toksičnost leţi u sposobnosti bioakumulacije od strane primarnih producenata, posebice mikroalga, koje osiguravaju transport Se duţ hranidbenih lanaca u vodenim ekosustavima. U kontekstu navedenog, cilj istraţivanja bio je utvrditi učinak različitih koncentracija selenita (5, 25, 50 i 100 mg/L) na rast kulture jednostanične zelene alge Monoraphidium cf. contortum, aktivnost antioksidacijskog enzima glutation S-trasferaze (GST), sadrţaj ukupnih topljivih fenola i ukupnog glutationa (tGSH) te ukupan sadrţaj reaktivnih kisikovih tvari (ROS) tijekom različitih vremenskih perioda izlaganja (24, 48 i 72 sata). Tretmani većim koncentracijama Se rezultirali su inhibicijom rasta alga i pojačanim stvaranjem ROS-a u stanicama. TakoĎer, selenit je uzrokovao povećanje ukupne koncentracije topljivih fenola, smanjenje koncentracije tGSH te povećanje aktivnosti GST-a. Tretman najniţom koncentracijom selenita imao je pozitivan učinak na rast alga i smanjenje sadrţaja ukupnog ROS-a u stanicama. Učinak Se na antioksidacijski odgovor ovisio je o primijenjenoj koncentraciji i vremenu izlaganja selenitu.
Abstract (english)
Selenium (Se) is a trace element that appears in nature in many organic and inorganic forms. Previous investigations showed a dual impact of Se on the living world - it acts as an essential micronutrient, but also as a toxicant, depending on its concentration, chemical form and other environmental parameters. Potential toxicity lies in the ability of its bioaccumulation by primary producers, particularly microalgae, which provide its transport along the food chains in aquatic ecosystems. In the context of the above, the study aimed to determine the effect of different selenite concentrations (5, 25, 50 and 100 mg/L) on the growth of unicellular green alga Monoraphidium cf. contortum, the activity of antioxidant enzyme glutathione Stransferase (GST), the content of total soluble phenols and total glutathione (tGSH) and the total content of reactive oxygen species (ROS) during different periods of treatment (24, 48 and 72 hours). The results showed the inhibiting effect of higher Se concentrations on algal growth and enhanced ROS generation in cells. Also, selenite treatment induced an increase in the content of total soluble phenols, a decrease in the content of tGSH and increased GST activity. Treatment with the lowest concentration of selenite had a positive effect on algal growth and reduced the total content of ROS in cells. The effect of Se on the antioxidative response in algal cells depended on the applied concentration and time of exposure to selenite.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:170025
Study programmeTitle: Master of Science Graduate University Study Programme in Biology and Chemistry; specializations in: Master Education In Chemistry and Biology
Course: Master Education In Chemistry and Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-03-12 12:22:08