prikaz prve stranice dokumenta Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi
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master's thesis
Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. urn:nbn:hr:181:224061
Boljevac, Kristina
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

Cite this document

Boljevac, K. (2022). Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:224061

Boljevac, Kristina. "Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:224061

Boljevac, Kristina. "Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2022. https://urn.nsk.hr/urn:nbn:hr:181:224061

Boljevac, K. (2022). 'Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:224061

Boljevac K. Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2022 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:181:224061

K. Boljevac, "Analiza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:181:224061

Metadata
TitleAnaliza regije uz 3' kraj MAT lokusa izolata gljive Ophistoma novo-ulmi
Title (english)Analysis of the 3'-flanking region of the MAT locus from Ophiostoma-novo ulmi isolates
AuthorKristina Boljevac
MentorZorana Katanić (mentor)
MentorLjiljana Krstin (komentor)
Committee memberRosemary Vuković (predsjednik povjerenstva)
Committee memberZorana Katanić (član povjerenstva)
Committee memberIvna Štolfa Čamagajevac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2022-10-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Značajni čimbenici u evolucijskom razvoju fitopatogene gljive Ophiostoma novo-ulmi, uzročnika holandske bolesti brijesta, su hibridizacija i prijenos gena od srodne vrste O. ulmi. Spolno razmnožavanje, koje značajno doprinosi povećanju varijabilnosti O. novo-ulmi, moguće je samo između različitih tipova parenja, označenih kao MAT-1 i MAT-2, što je određeno različitim idiomorfima na MAT lokusu. Pojava MAT-1 tipa u originalno klonalnim populacijama O. novo-ulmi MAT-2 tipa dogodila se primarno putem introgresije gena vrste O. ulmi u genom O. novo-ulmi. U ovom radu analizirani su izolati O. novo-ulmi uzorkovani 2012. i 2021. godine na lokalitetima Jastrebarsko i Nova Kapela. Cilj je bio analizirati regiju uz 3' kraj MAT lokusa te istražiti jesu li oba MAT tipa prisutna u analiziranim populacijama. Sekvenciranje regije uz 3' kraj MAT lokusa izolata O. novo-ulmi ukazalo je na introgresiju MAT lokusa kod dijela analiziranih izolata. Dokazana su oba tipa spolne kompatibilnosti (MAT-1 i MAT-2) što ukazuje na postojanje potencijala za spolno razmnožavanje. Do značajnijeg širenja MAT-1 izolata na istraženim lokalitetima na području Hrvatske došlo je nakon 2012. godine.
Abstract (english)
Significant factors in the evolutionary development of the phytopathogenic fungus Ophiostoma novoulmi, the causative agent of Dutch elm disease, are hybridization and gene transfer from the related species O. ulmi. Sexual reproduction, which significantly increases the variability of O. novo-ulmi, is possible only between different mating types, designated as MAT-1 and MAT-2, which are determined by different idiomorphs at the MAT locus. The appearance of MAT-1 type in originally clonal populations of O. novo-ulmi of MAT-2 type occurred primarily through introgression from O. ulmi. In this study, O. novo-ulmi isolates sampled in 2012 and 2021 at the sites Jastrebarsko and Nova Kapela were analyzed. The aim was to analyze 3’ flanking region of the MAT locus and to investigate whether both MAT types are present in the analyzed populations. By sequencing 3’ flanking region of the MAT locus, introgression of MAT locus was proven for the same of the analyzed isolates. The presence of both mating types has also been proven, which indicates the potential for the sexual reproduction of the pathogen. A significant spread of MAT-1 isolates at investigated sites occurred after 2012.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:224061
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2022-11-02 10:27:36