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master's thesis
Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. urn:nbn:hr:181:081536
Kerezović, Sanja
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

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Kerezović, S. (2023). Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:081536

Kerezović, Sanja. "Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:081536

Kerezović, Sanja. "Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2023. https://urn.nsk.hr/urn:nbn:hr:181:081536

Kerezović, S. (2023). 'Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:081536

Kerezović S. Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:181:081536

S. Kerezović, "Učinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:181:081536

Metadata
TitleUčinak fitohormona, nedostatka dušika i povišenog saliniteta na produktivnost slatkovodne alge Parachlorella kessleri
Title (english)Effect of phytohormones, nitrogen deficiency, and high salinity on productivity of the freshwater algae Parachlorella kessleri
AuthorSanja Kerezović
MentorVesna Peršić (mentor)
MentorJasenka Antunović Dunić (komentor)
Committee memberMartina Varga (predsjednik povjerenstva)
Committee memberDubravka Špoljarić Maronić (član povjerenstva)
Committee memberJasenka Antunović Dunić (član povjerenstva)
Committee memberVesna Peršić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2023-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Regulatori rasta, nedostatak dušika i povišeni salinitet su čimbenici koji utječu na promjene u raspodjeli ugljika potičući akumulaciju škroba i lipida u slatkovodnim algama. U istraživanju je analiziran utjecaj predtretmana sintetskim regulatorom rasta citokininskog djelovanja, 6-benzilaminopurinom (6-BA), na produktivnost slatkovodne zelene alge Parachlorella kessleri izložene nutritivnom stresu. Nedostatak dušika povećao je akumulaciju škroba i ugljikohidrata (za 65 i 68 %) u algama, ali smanjio udio lipida (za 66 %), pigmenata (81 %) i proteina (za 69 %), povećavajući rizik od oksidativnog stresa. Međutim, prethodno izlaganje 6-BA ublažilo je neke od negativnih učinaka nedostatka dušika, posebice u pogledu očuvanja sadržaja lipida. Povećanje koncentracije NaCl također je utjecalo na raspodjelu ugljika, potičući akumulaciju lipida (povećanje za 45 %), pigmenata (do 28 %) i proteina (do 63 %), ali bez stvaranja oksidativnog stresa što je rezultat sposobnosti stanica algi da u blažim stresnim uvjetima povećaju proizvodnju pigmenata radi povećanja fotosintetske učinkovitosti, ali i antioksidativnih enzima kako bi se zaštitile od oštećenja. Prema tome, slatkovodna zelena alga P. kessleri predstavlja obećavajući izvor za proizvodnju biogoriva zbog svoje sposobnosti akumulacije lipida i škroba, no ukupni utjecaj na produktivnost može varirati ovisno o uvjetima uzgoja te su za širu komercijalnu primjenu potrebna daljnja istraživanja metaboličkih procesa u navedenim uvjetima.
Abstract (english)
Certain variables such as growth regulators, nitrogen deficits, and high salinity influence carbon distribution changes by promoting starch and lipids accumulation in freshwater algae. The study examined the impact of pre-treatment using 6-benzylaminopurine (6-BA), a synthetic growth regulator, on the productivity of Parachlorella kessleri, a freshwater green alga, when subjected to nutritional stress. Nitrogen deficiency increased starch accumulation (by 65%) but decreased the proportion of lipids (by 66%), pigments (81%), and proteins (by 69%), increasing the risk of oxidative stress. However, pre-exposure to 6-BA mitigated some of the nitrogen deficiency's adverse effects, particularly in preserving lipid content. An increase in NaCl concentration influenced carbon allocation in algal cells, resulting in a rise in lipid (by 45%), pigment (by 28%), and protein (by 63%) accumulation. Despite these changes, no oxidative stress was observed. This is attributed to the algal cells' adaptive response to mild stress, which increases pigment production to improve photosynthetic efficiency and increase antioxidant enzyme levels for protection against damage. In conclusion, the freshwater green alga P. kessleri is a promising source of biofuel production due to its ability to accumulate lipids and starch; however, the overall impact on productivity may vary depending on cultivation conditions and further research into metabolic processes under the mentioned conditions is required for more comprehensive commercial application.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:081536
Study programmeTitle: Master of Science Graduate University Study Programme in Nature and Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra Zaštite priode i okoliša (magistar/magistra Zaštite priode i okoliša)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2023-10-16 09:24:28