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master's thesis
AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2014. urn:nbn:hr:181:789106
Horvat, Martina
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

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Horvat, M. (2014). AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:789106

Horvat, Martina. "AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2014. https://urn.nsk.hr/urn:nbn:hr:181:789106

Horvat, Martina. "AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2014. https://urn.nsk.hr/urn:nbn:hr:181:789106

Horvat, M. (2014). 'AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:789106

Horvat M. AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2014 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:181:789106

M. Horvat, "AKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:181:789106

Metadata
TitleAKTIVNOST MIKROBNE DEHIDROGENAZE PRI RAZLIĈITIM TIPOVIMA TLA I BILJNOG POKROVA
Title (english)MICROBIAL DEHYDROGENASE ACTIVITY UNDER DIFFERENT TYPES OF SOIL AND PLANT COVER
AuthorMartina Horvat
MentorGoran Palijan (mentor)
Committee memberGoran Palijan (predsjednik povjerenstva)
Committee memberIvna Štolfa Čamagajevac (član povjerenstva)
Committee memberAlma Mikuška (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2014-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U tlu postoji mnoštvo enzima od kojih svaki ima kljuĉnu biokemijsku ulogu u konaĉnom procesu pretvorbe energije. Kao indikator ukupne biološke aktivnosti u tlu, najĉešće se koristi aktivnost dehidrogenaza. To su unutarstaniĉni enzimi i glavni predstavnici razreda oksidoreduktaza. Dehidrogenaze sudjeluju u biološkoj oksidaciji organske tvari tako što prenose elektrone iprotone s organskih supstanci na anorganske akceptore. Mikrobiološka oksidacija organske tvari povezana je s membranski vezanim lancem proteina za transport elektrona koji koristi molekule kisika kao konaĉne elektron akceptore te je povezan s sintezom ATP-a pomoću procesa oksidacijske fosforilacije. Kako su elektroni stalno u pokretu (preko elektron transportnog lanca), umjesto na kisik oni se primaju na topive tetrazolijeve soli kao što je INT [2 (jod - fenil) - 3 (nitro - fenil) - 5 (feril) tetrazolium klorid)] uz stvaranje crveno obojanog produkta (INT formazan) koji je netopiv u vodi. U vodi netopivi produkt se može odrediti spektrofotometrijski nakon ekstrakcije s organskim otapalom. Mjerenje dehidrogenazne aktivnosti koristeći tetrazolijeve soli razliĉito je za svaki tip tla i ovisi o mnoštvu okolišnih ĉimbenika tla. Cilj ovog rada bio je utvrditi postoje li razlike u dehidrogenaznoj aktivnosti između dva razliĉita tipa tla (ĉernozema i luvisola) te na 3 vrste biljnog pokrova (suncokret, kukuruz i šumsko tlo). Također, želi se utvrditi postoji li razlika u enzimskoj aktivnosti površinskog i dubinskog sloja tla s obzirom na mjerene okolišne ĉimbenike tla kao što su pH, koliĉina vlage, elektriĉni konduktivitet te koliĉina organske tvari u tlu. Dobiveni rezultati pokazuju da je dehidrogenazna aktivnost kod uzoraka tla sa površine znatno veća nego kod uzoraka tla uzorkovanih iz dubokog sloja. Također utvrđena je statistiĉki znaĉajna interakcija mjerenih faktora (tip tla, tip biljnog pokrova i tip uzorka) koja se odražava na okolišnim ĉimbenicima tla.
Abstract (english)
There are many different enzymes in soil, and each of them has a specific function in the biogeochemical cycles and energy transformation. As an indicator of overall biological activity in the soil, the most commonly used method is the determination of dehydrogenase acitivity. Dehydrogenase activity can be considered to be a good measure of microbial oxidative activity in soils. It is usually determined by measuring the amount of an artificial electron acceptor reduced by microbial activity, such as a soluble tetrazolium salt with a red colored reduced form (formazan) that can be determined colorimetrically following extraction with a suitable solvent. This method of determining dehydrogenase activity using tetrazolium salts differs for each type of soil and it depends on various environmental factors. The aim of this study was to determine wether there are any differences in the dehydrogenase activity between two different types of soil (chernozem and luvisol) and three types of plant cover (corn, sunflower and forest soil). Also we wanted to determine wether there is a difference in the enzyme activity for surface soil samples and the subsoil samples considering various environmental factors such as pH, moisture, electrical conductivity and the amount of organic matter in the soil. The results showed that there is in fact a difference in the dehydrogenase activity between the 2 types of soil samples (surface and subsoil). Enzyme activity in the surface is far greater that in the subsoil. Also, statisticaly significant interactions have been found between all the measured factors (type of soil, type of plant cover and type of sample) that consequently reflects on all environmental variables.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:789106
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2017-01-23 08:27:26