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master's thesis
Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)
Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2015. urn:nbn:hr:181:824788
Mihić, Ivan
Josip Juraj Strossmayer University of Osijek
Department of biology

Institutional repository: Repository of Department of biology, Josip Juraj Strossmayer University of Osijek

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Mihić, I. (2015). Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:824788

Mihić, Ivan. "Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2015. https://urn.nsk.hr/urn:nbn:hr:181:824788

Mihić, Ivan. "Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2015. https://urn.nsk.hr/urn:nbn:hr:181:824788

Mihić, I. (2015). 'Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:181:824788

Mihić I. Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2015 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:181:824788

I. Mihić, "Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:181:824788

Metadata
TitleOdređivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)
Title (english)Sex determination in herons (Ardeidae) using CHD gene
AuthorIvan Mihić
MentorAlma Mikuška (mentor)
Committee memberLjiljana Krstin (predsjednik povjerenstva)
Committee memberIvna Štolfa Čamagajevac (član povjerenstva)
Committee memberAlma Mikuška (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of biology
Osijek
Defense date and country2015-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Spolni dimorfizam kod mladih jedinki ĉaplji nije izražen, te je jedna od pouzdanih metoda utvrđivanja spola mladih ptica putem molekularnih biljega. Cilj diplomskog rada je usporedba metoda za izolaciju DNA iz pera i ljuski jaja ĉaplji, te utvrđivanje spola prstenovanih jedinki ĉaplji. Rezultati pokazuju da je najveći prinos DNK dobiven iz pera koja su u razvoju (prokrvljena pera) i ljuski jaja. Prinos DNA je bio manji kad se koristio komercijalni kit za izolaciju u odnosu na izolaciju standardnim protokolom. Određen je spol deset prstenovanih ĉaplji na ribnjacima Jasinje. U dva gnijezda ĉaplje dangube (Ardea purpurea) utvrđeno je ukupno šest mužjaka(tri po gnijezdu), dok su u dva gnijezda velike bijele ĉaplje (Ardea alba) utvrđene ĉetiri ženke, tri iz jednog i jedna iz drugog gnijezda. Ovo je prvo utvrđivanje spola ĉaplji putem CHD gena u Hrvatskoj. Opsežnijim višegodišnjim istraživanjima mogao bi se utvrditi odnos spolova u populaciji, stabilnost populacije, te poduzeti potrebne mjere zaštite gnijezdeće populacije ĉaplji u Hrvatskoj.
Abstract (english)
In sexually monomorphic species like herons one of the most reliable method of sex determination in young birds are molecular markers. The aim this work is a comparison of methods for the isolation of DNA from feathers and eggshells in herons, and sex determination of ringed young birds. Results showed that the highest yield of DNA was obtained from the young chick feathers, which are in development, and egg shells. The yield of DNA was lower when a commercial kit was used for isolation compared to the isolation with the standard protocol. Sex-typing was performed in ten ringed herons in the fishponds Jasinje. In two nests of purple heron(Ardea purpurea) a total of six males (three per nest) were found while in two nests of the great white egret(Ardea alba) we identified four females, three in one and one in another nest. This is the first time in Croatia that molecular approach was used for sex determination in herons by using CHD gene. More extensive research trough years would determine sex ratio in the population, population stability, and to lead to the necessary measures in order to protect nesting population of herons in Croatia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:181:824788
Study programmeTitle: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Course: Master of Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-01-24 09:00:10