prikaz prve stranice dokumenta Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
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Scientific paper - Original scientific paper
Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
Photodiagnosis and Photodynamic Therapy, 15 (2016); 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003
Malatesti, Nela; Harej, Anja; Kraljević Pavelić, Sandra; Lončarić, Martin; Zorc, Hrvoje; Wittine, Karlo; Anđelković, Uroš; Josić, Đuro

Institutional repository: Repository of the University of Rijeka, Faculty of Biotechnology and Drug Development

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Malatesti, N., Harej, A., Kraljević Pavelić, S., Lončarić, M., Zorc, H., Wittine, K. ... Josić, Đ. (2016). Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. Photodiagnosis and Photodynamic Therapy, 15., 115-126. doi: 10.1016/j.pdpdt.2016.07.003

Malatesti, Nela, et al. "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate." Photodiagnosis and Photodynamic Therapy, vol. 15, 2016, pp. 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003

Malatesti, Nela, Anja Harej, Sandra Kraljević Pavelić, Martin Lončarić, Hrvoje Zorc, Karlo Wittine, Uroš Anđelković and Đuro Josić. "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate." Photodiagnosis and Photodynamic Therapy 15 (2016): 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003

Malatesti, N., et al. (2016) 'Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate', Photodiagnosis and Photodynamic Therapy, 15, pp. 115-126. doi: 10.1016/j.pdpdt.2016.07.003

Malatesti N, Harej A, Kraljević Pavelić S, Lončarić M, Zorc H, Wittine K, and sur.. Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. Photodiagnosis and Photodynamic Therapy [Internet]. 2016 [cited 2025 March 14];15:115-126. doi: 10.1016/j.pdpdt.2016.07.003

N. Malatesti, et al., "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate", Photodiagnosis and Photodynamic Therapy, vol. 15, pp. 115-126, 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:193:798368. [Accessed: 14 March 2025]

Metadata
Title (english)Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
AuthorNela Malatesti
AuthorAnja Harej
AuthorSandra Kraljević Pavelić
AuthorMartin Lončarić
AuthorHrvoje Zorc
AuthorKarlo Wittine
AuthorUroš Anđelković
AuthorĐuro Josić
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract (english)
Photodynamic therapy (PDT) is a treatment that aims to kill cancer cells by reactive oxygen species, mainly singlet oxygen, produced through light activation of a photosensitiser (PS). Amongst photosensitisers that attracted the most attention in the last decade are cationic and amphiphilic molecules based on porphyrin, chlorin and phthalocyanine structures. Our aim was to join this search for more optimal balance of the lipophilic and hydrophilic moieties in a PS. A new amphiphilic porphyrin, 5-(4-octadecanamidophenyl)-10, 15, 20- tris(N-methylpyridinium-3-yl)porphyrin trichloride (5) was synthesised and characterised by 1H NMR, UV–vis and fluorescence spectroscopy, and by MALDI-TOF/TOF spectrometry. In vitro photodynamic activity of 5 was evaluated on HeLa cell lines and compared to the activity of the hydrophilic 5-(4- acetamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride (7). Low fluence rate (2 mW cm−2) of red light (643 nm) was used for the activation, and both porphyrins showed a drug dose-response as well as a light dose-response relationship, but the amphiphilic porphyrin was presented with significantly lower IC50 values. The obtained IC50 values for 5 were 1.4 μM at 15 min irradiation time and 0.7 μM when the time of irradiation was 30 min, while for 7 these values were 37 and 6 times higher, respectively. These results confirm the importance of the lipophilic component in a PS and show a potential for 5 to be used as a PS in PDT applications.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionSubmitted version
Journal titlePhotodiagnosis and Photodynamic Therapy
Numberingvol. 15, pp. 115-126
p-ISSN1572-1000
DOIhttps://doi.org/10.1016/j.pdpdt.2016.07.003
URN:NBNurn:nbn:hr:193:798368
Publication2016
Document URLhttp://bib.irb.hr/830506
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2018-09-13 15:42:10