prikaz prve stranice dokumenta EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats
  Download
PDF 247.78 KB
Scientific paper - Original scientific paper
EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1718 (2005), 1-2; 44-52. https://doi.org/10.1016/j.bbamem.2005.09.022
Zuvić-Butorac, Marta; Herak-Kramberger, M Carol; Krilov, Dubravka; Sabolić, Ivan; Herak, Janko

Institutional repository: Repository of the University of Rijeka, Faculty of Biotechnology and Drug Development

Cite this document

Zuvić Butorac, M., Herak Kramberger, M. C., Krilov, D., Sabolić, I. & Herak, J. (2005). EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1718. (1-2), 44-52. doi: 10.1016/j.bbamem.2005.09.022

Zuvić Butorac, Marta, et al. "EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats." Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1718, no. 1-2, 2005, pp. 44-52. https://doi.org/10.1016/j.bbamem.2005.09.022

Zuvić Butorac, Marta, M Carol Herak Kramberger, Dubravka Krilov, Ivan Sabolić and Janko Herak. "EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats." Biochimica et Biophysica Acta (BBA) - Biomembranes 1718, no. 1-2 (2005): 44-52. https://doi.org/10.1016/j.bbamem.2005.09.022

Zuvić Butorac, M., et al. (2005) 'EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats', Biochimica et Biophysica Acta (BBA) - Biomembranes, 1718(1-2), pp. 44-52. doi: 10.1016/j.bbamem.2005.09.022

Zuvić Butorac M, Herak Kramberger MC, Krilov D, Sabolić I, Herak J. EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats. Biochimica et Biophysica Acta (BBA) - Biomembranes [Internet]. 2005 December [cited 2024 May 05];1718(1-2):44-52. doi: 10.1016/j.bbamem.2005.09.022

M. Zuvić Butorac, M. C. Herak Kramberger, D. Krilov, I. Sabolić and J. Herak, "EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats", Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1718, no. 1-2, pp. 44-52, December 2005. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:193:688943. [Accessed: 05 May 2024]

Metadata
Title (english)EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats
AuthorMarta Zuvić-Butorac
AuthorM Carol Herak-Kramberger
AuthorDubravka Krilov
AuthorIvan Sabolić
AuthorJanko Herak
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Biophysics and Medical Physics
Abstract (english)
Numerous studies have demonstrated various structure/function correlations at the level of transport proteins in the kidney cell membranes and various intracellular organelles. However, characterization of the lipid phase of these membranes is rare. Here, we report the differences in lipid organization and dynamics of the brush-border membranes (BBM), basolateral membranes (BLM) and endocytotic vesicles (EV), isolated from the kidney cortex of intact rats, studied with the EPR spectroscopy of the spin-labeled membrane lipids. The EPR spectra were analyzed by comparing experimentally observed line shapes with the line shapes calculated according to the theoretical model developed for liquid crystals. In the fitting procedure, three different lipid domains were assumed, which revealed clear differences in the lipid ordering and rotational correlation times, as well as in the lipid partition of these domains in each of the three types of membranes. A similar approach, used to compare the spectroscopic characteristics of BBM from control and cadmium-intoxicated rats, showed significantly changed ordering and increased molecular mobility in the lipid phase of BBM from Cd-treated animals. As tested by an established fluorescence assay, the Cd-induced changes in the lipid mobility co localized with approximately 5-fold higher conductance of BBM for potassium, with unchanged conductance for protons.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBiochimica et Biophysica Acta (BBA) - Biomembranes
Numberingvol. 1718, no. 1-2, pp. 44-52
p-ISSN0005-2736
DOIhttps://doi.org/10.1016/j.bbamem.2005.09.022
URN:NBNurn:nbn:hr:193:688943
Publication2005-12
Document URLhttps://www.sciencedirect.com/science/article/pii/S0005273605003135?via%3Dihub
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-09-30 17:55:40