prikaz prve stranice dokumenta Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora
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master's thesis
Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora
Rijeka: University of Rijeka, 2021. urn:nbn:hr:193:004312
Marić, Katharina
University of Rijeka
Faculty of Biotechnology and Drug Development

Institutional repository: Repository of the University of Rijeka, Faculty of Biotechnology and Drug Development

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Marić, K. (2021). Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:004312

Marić, Katharina. "Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora." Master's thesis, University of Rijeka, 2021. https://urn.nsk.hr/urn:nbn:hr:193:004312

Marić, Katharina. "Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora." Master's thesis, University of Rijeka, 2021. https://urn.nsk.hr/urn:nbn:hr:193:004312

Marić, K. (2021). 'Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora', Master's thesis, University of Rijeka, accessed 12 May 2024, https://urn.nsk.hr/urn:nbn:hr:193:004312

Marić K. Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora [Master's thesis]. Rijeka: University of Rijeka; 2021 [cited 2024 May 12] Available at: https://urn.nsk.hr/urn:nbn:hr:193:004312

K. Marić, "Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora", Master's thesis, University of Rijeka, Rijeka, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:193:004312

Metadata
TitleRazvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora
Title (croatian)Razvoj inovativnog phage display protokola za probir antitijela za unaprijeđenje rane detekcije tumora
AuthorKatharina Marić
MentorSandra Kraljević Pavelić (mentor)
MentorMirela Sedić (komentor)
Committee memberElitza Petkova Markova - Car (predsjednik povjerenstva)
Committee memberDean Marković (član povjerenstva)
Committee memberSandra Kraljević Pavelić (član povjerenstva)
Committee memberMirela Sedić (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2021-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Advantages such as faster and cheaper production of highly specific engineered antibodies by phage display in vitro method enabled application of these antibodies for diagnostic or treatment purpose for many diseases including cancer. The goal of this master thesis was to develop an innovative phage display protocol for antibody screening, which will be applied for improvement of early cancer detection. Briefly, the developed protocol relies on a linear amplification of a donor’s antibody coding sequences, allowing to prepare a phage bank which is an exact molecular fingerprint of the donor’s antibodies repertoire. This protocol differs from traditional phage display banks preparation which rely on exponential amplification, selecting the most represented sequences and leading to a biased phage bank. Plasmids strands were converted to RNA, needed for the future preparation of the functional phagemid. Sequences were then combined with plasmid's strands, and the resulting phagemid was inserted in bacterias for positive clones screening. The perspective of this work will be to optimize the protocol and construct an antibody phage library with a high diversi
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:193:004312
Study programmeTitle: Drug research and development
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2021-10-27 09:38:19