Title Ekstrakcija i kvantifikacija metabolita iz lišća masline s područja Krka i Marčeljeve Drage Title (english) Extraction and quantification of metabolites from olive leaves obtained from Krk Island and Marčeljeva Draga Author Aida Hajdo Mentor Dean Marković (mentor)Committee member Karlo Wittine (predsjednik povjerenstva)Committee member Dean Marković (član povjerenstva)Committee member Sandra Kraljević Pavelić (član povjerenstva)Granter University of Rijeka Defense date and country 2017-09-29, Croatia Scientific / art field, BIOTECHNICAL SCIENCES Abstract Ekstrakt lišća masline naširoko je proučavan zbog svog antioksidativnog,
protuupalnog i antimikrobnog učinka te brojnih ostalih protektivnih svojstava, za
koje se smatra da su zaslužne u njemu prisutne fenolne komponente kao što su
oleuropein, 3-hidroksitirosol, tirosol, p-kumarinska kiselina, p-hidroksifeniloctena
kiselina, pinorezinol i mnoge druge. Cilj ovog rada bio je dokazati prisutnost
spomenutih komponenti i usporediti njihovu zastupljenost u maslinovom lišću s
Krka i Marčeljeve Drage, te usporediti utjecaj različitih metoda ekstrakcije na
ukupni sadržaj dobivenog ekstrakta. Korištene su tri metode ekstrakcije krutotekuće
uz: 80%-tni etanol pri 70 °C, 80%-tni etanol pri temperaturi vrelišta (78
°C) i destiliranu vodu pri 90 °C. Za identifikaciju i kvantifikaciju komponenti
korištena je kromatografija ultra visoke djelotvornosti (UHPLC).
Rezultati su ukazali na veću zastupljenost oleuropeina, tirosola, 3-
hidroksitirosola i 3-p-kumarinske kiseline u lišću mladih maslina iz Marčeljeve
Drage, dok je lišće starih maslina s područja Krka imalo veće koncentracije phidroksifeniloctene
kiseline i pinorezinola. 80%-tni etanol se pokazao
najpogodnijim otapalom za ekstrakciju oleuropeina i ostalih fenolnih komponenti
kod ekstrakta iz Marčeljeve Drage, dok su kod maslinovog lišća s Krka dobiveni
kontradiktorni rezultati koji ukazuju na bolje prinose s destiliranom vodom kao
otapalom. Sadržaj ekstrakta nije pokazao značajnu razliku ovisno o temperaturi
pri kojoj je provođena ekstrakcija za većinu fenolnih komponenti, osim kod
oleuropeina u lišću iz Marčeljeve Drage koji je u većim količinama bio prisutan pri
ekstraciji s etanolom pri 70 °C, nego što je bio pri 78 °C. Analiziran je i
komercijalni ekstrakt te je u njemu dokazana prisutnost oleuropeina. Potrebno
je nabaviti druge standarde i provesti analizu spektrometrijom masa kako bi
identificirale ostale prisutne komponente za koje nismo imali potrebne standarde.
Metode ekstrakcije korištene u ovom radu koriste zelena otapala,
jednostavne su, jeftine i daju visoke prinose fenolnih komponenti, zbog čega
pokazuju potencijal za primjenu u farmaceutskoj, kozmetičkoj i prehrambenoj
industriji.
Abstract (english) Olive leaf extract has been widely studied because of its antioxidative,
anti-inflammatory, and antimicrobial effect, as well as other protective properties
that are believed to be present due to the abundance of components such as
oleuropein, 3-hydroxytyrosol, tyrosol, p-coumaric acid, p-hydroxyphenyl acetic
acid, pinoresinol and many others. This study aimed to prove the presence of
the above-mentioned compounds in olive leaf extracts from two different
geographical origins; Marčeljeva Draga and Krk, and to compair their total
concentrations. The second goal was to compare the effect of three different
solid-liquid extraction methods on total phenolic extract content, for which we
used 80% ethanol at 70 °C and 78 °C, respectively, and distilled water at 90°C.
Ultra-high performance liquid chromatography was used for the identification and
quantifiaction of compounds.
The results showed a higher cocentration of oleuropein, tyrosol, 3-
hydroxytyrosol and p-coumaric acid in young olives leaves from Marčeljeva
Draga, while older olive leaves from Krk had higher concentrations of phydroxyphenyl
acetic acid and pinoresinol. 80% ethanol proved to be the most
suitable solvent for the extraction of oleuropein and other phenolic components
in the extract from Marčeljeva Draga, while the extract from Krk gave
contradictory results; better yields using water as a solvent. The extract content
did not show a significant difference depending on the temperature at which
extraction was performed for most phenolic components, except for oleuropein in
leaves from Marčeljeva Draga, which was present in larger quantities when
extracted with ethanol at 70 °C, opposed to boiling at 78 °C. A commercial olive
leaf extract was also analyzed and the presence of oleuropein was confirmed.
The extraction methods used in this research require green solvents, they
are simple, inexpensive and give high levels of phenolic components, which is
why they show potential for usage in pharmaceutical, cosmetic and food
industries.
Keywords
lišće masline
ekstrakcija
kvantifikacija
fenolne komponente
UHPLC
Keywords (english)
olive leaves
extraction
quantification
phenolic compounds
UHPLC
Language croatian URN:NBN urn:nbn:hr:193:054452 Study programme Title: Medicinal chemistry Type of resource Text File origin Born digital Access conditions Access restricted to students and staff of home institution Terms of use Created on 2017-11-03 10:57:03
