The results of epidemiological studies clearly indicate the beneficial effects of moderate wine consumption on human health, especially on the cardiovascular system. Wine slows the development of atherosclerosis and arteriosclerosis by achieving anti-inflammatory, antiplatelet and antioxidant effects. Except alcohol, wine polyphenols are thought to mediate these effects. Traditional macerated white wines with rich phenolic content attract the attention of producers and scientists, and their biological effects are less well examined. The aim of the study was to examine and compare the impact of moderate consumption of standard and macerated white wine on the vasodilation and vasoconstriction response of the rat aorta. Male Sprague Dawley rats (N = 45) were randomized into 3 groups and subjected to a 4-week consumption protocol: the control (K) group consumed only water, the SV group standard, and the MV macerated white wine. Following consumption trial, the rat thoracic aortas were isolated and the vascular rings prepared. The rings of all groups (N = 107) were additionally randomized and exposed to one of two in vitro dose - response protocols: vasodilatory protocol where the precontracted rings were treated with acetylcholine and vasoconstriction protocol, without precontraction with noradrenaline. Acetylcholine and noradrenaline were applied cumulatively to acomplish final concentrations from 1nM to 10 µM in the organ baths. The main outcome measures were the maximal vasodilation and vasoconstriction effect (Emax) and the concentration of noradrenaline and acetylcholine that produce 50% of maximal effect (EC50). Noradrenaline-induced vasoconstriction effect was similar for all groups. However, the consumption of macerated white wine changed the vasodilation efficacy of acetylcholine, which induced a significantly greater vasodilatory effect of MV rat aorta in the concentration range from 0.1 to 1 µM. Accordingly, the potency of acetylcholine was the highest in MV, slightly lower in SV, and the lowest in the K group with corresponding EC50 values of 43 nM, 57 nM and 70 nM for MV, SV and K, respectively. The obtained results indicate a vasoprotective effect of moderate consumption of macerated white wine in rats. The effect of standard white wine consumption needs to be further clarified.