prikaz prve stranice dokumenta Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness
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master's thesis
Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness
Rijeka: University of Rijeka, 2022. urn:nbn:hr:193:852173
Bergman, Mihaela
University of Rijeka
Faculty of Biotechnology and Drug Development

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Bergman, M. (2022). Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:852173

Bergman, Mihaela. "Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness." Master's thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:852173

Bergman, Mihaela. "Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness." Master's thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:852173

Bergman, M. (2022). 'Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness', Master's thesis, University of Rijeka, accessed 12 May 2024, https://urn.nsk.hr/urn:nbn:hr:193:852173

Bergman M. Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness [Master's thesis]. Rijeka: University of Rijeka; 2022 [cited 2024 May 12] Available at: https://urn.nsk.hr/urn:nbn:hr:193:852173

M. Bergman, "Stress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness", Master's thesis, University of Rijeka, Rijeka, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:193:852173

Metadata
TitleStress as a factor affecting aggregation of NPAS3 and other proteins related to mental illness
Title (english)Stres kao faktor utjecaja na agregaciju NPAS3 i ostalih proteina prisutnih u mentalnim bolestima
AuthorMihaela Bergman
MentorNicholas Bradshaw (mentor)
Committee memberRozi Andretić Waldowski (predsjednik povjerenstva)
Committee memberŽeljka Maglica (član povjerenstva)
Committee memberNicholas Bradshaw (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2022-07-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Chronic mental illness are some of the leading cause of disability worldwide. They include major depressive disorder, bipolar disorder and schizophrenia. Schizophrenia has the most severe symptoms, with an estimated prevalence of 24 million people in the general population. Chronic mental illness are characterized by both genetic and non-genetic risk factors, however, their underlying biological cause remains relatively unknown. Several candidates have been implicated in protein aggregation pathology of chronic mental illness, including TRIOBP-1, DISC1, CRMP1 and NPAS3. In this thesis, we investigate the effect of environmental stress factors on aggregation of these proteins, when into cultured cells. Initially we were focusing on treatment protocol optimization and settled on stress treatment for 3 hours. The results suggest that TRIOBP-1, DISC1 and NPAS3 show signs of aggregation when following application of stress. However, NPAS3 showed the most interesting results, where it clearly mislocalized from nuclei to the cytoplasm forming insoluble aggregates. The tests were repeated, showing aggregation of NPAS3 after it was treated with sodium arsenide, iron (II) chloride or zinc acetate, as well as potential aggregation when treated with calcium chloride and an MG132 proteasome inhibitor. Environmental stress factors potentially do affect protein aggregation, which can be used as a new approach to investigate the effect on chronic mental illness. Further cell culture research could provide us with initial results and could be used as a model for observing stress in chronic mental illness based on these findings. Further research should be focused on stress treatment protocol modification, as well as the implication of other stress factors, such as heat shock, as the high temperature resulted in a quantitative test failure, where most of the transfected cells showed signs of NPAS3 aggregation.
Abstract (croatian)
Kronične mentalne bolesti jedan su od vodećih uzroka smetnji širom svijeta. One uključuju depresivni poremećaj, bipolarni poremećaj i shizofreniju. Shizofreniju karakteriziraju nateži simptomi, te je procijenjena prevalencija kod 24 miljuna ljudi u populaciji. Kronične mentalne bolesti karakteriziraju genski faktori i faktori koji nemaju genski uzrok, međutim, njihov temeljni biološki uzrok ostaje relativno nepoznat. Nekoliko kandidata povezanih s patologijom agregacije proteina kod mentalnih bolesti uključuju TRIOBP-1, DISC1, CRMP1 i NPAS3. U ovom diplomskom radu istražujemo učinak stresnih faktora okoliša na agregaciju ovih proteina u staničnim kulturama. U početku smo se usredotočili na optimizaciju protokola za tretiranje stanica stres faktorima i odlučili smo se na tretiranje u trajanju od 3 sata. Rezultati ukazuju da TRIOBP-1, DISC1 i NPAS3 agregiraju nakon tretiranja stres faktorima. Međutim, NPAS3 je pokazao najzanimljivije rezultate, gdje se jasno vidi premještaj iz jezgre stanice u citoplazmu, stvarajući netopljive agregate. Ponovljena testiranja potvrđuju NPAS3 aggregaciju nakon tretiranja s natrijevim arsenidom, željezovim (II) kloridom i cink acetatom, kao i potencijalnu agregaciju nakon tretiranja kalcijevim kloridom i inhibitorom proteasoma MG132. Okolišni stres faktori potencijalno utječu na agregaciju proteina, te se mogu koristiti kao novi pristup istraživanja učinka stres faktora na kronične mentalne bolesti. Daljnja istraživanja na staničnim kulturama mogla bi nam dati početne rezultate i koristiti se kao model za promatranje učinka stresa kod kroničnih mentalnih bolesti na temelju ovih otkrića. Daljenja istraživanja bi trebala biti usmjerena na modifikaciju protokola za tretiranje stanica, kao i korištenje drugih faktora stresa, primjerice toplinski šok, obzirom da je visoka temperatura rezultirala neuspješnim kvantitativnim eksperimentom, gdje je većina transficiranih stanica pokazivala znakove NPAS3 agregacije.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:193:852173
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-28 11:50:16