Title Povezanost redoks parametara kod selektiranih sojeva Drosophila melanogaster s visokom i niskom preferencijom za metamfetamin
Title (english) Connection of redox parameters in selected strains Drosophila melanogaster with high and low preference for metamphetamine
Author Ivona Starčević
Mentor Rozi Andretić Waldowski (mentor)
Mentor Ana Filošević Vujnović (komentor)
Committee member Rozi Andretić Waldowski (predsjednik povjerenstva)
Committee member Ana Filošević Vujnović (član povjerenstva)
Committee member Jelena Ban (član povjerenstva)
Committee member Stribor Marković (član povjerenstva)
Committee member Ivana Munitić (član povjerenstva)
Granter University of Rijeka (Faculty of Biotechnology and Drug Development) Rijeka
Defense date and country 2023-06-28, Croatia
Scientific / art field, discipline and subdiscipline BIOTECHNICAL SCIENCES Biotechnology
Abstract Metamfetamin (METH) je psihostimulans koji se široko zlouporabljuje
diljem svijeta. Sve je veća prevalencija ovisnosti koja može potencijalno biti
povezana kod potomaka čiji su roditelji bili ovisni što potomke čini rizičnom
skupinom. METH u organizmu djeluje na morfološke i funkcionalne promjene
neuralnih mreža – neuralnu plastičnost. Naš model za istraživanje neuralne
plastičnosti je samoadministracija METH-a koju koristimo za provedbu
umjetne selekcije kako bi istražili neurobiološku osnovu razlike u visokoj (HP)
i niskoj (LP) preferenciji.
Cilj ovog eksperimentralnog rada bio je utvrditi hoće li preferencijalna
samoadministracija METH-a pokazati promjene u redoks parametrima kod F1
generacije potomaka 22. i 28. generacije HP i LP soja, te hoće li ti redoks
parametri biti stabilni kod F2 i F3 potomaka 22. i 28. generacije HP i LP sojeva
nakon prestanka selekcije.
Dobiveni rezultati prikazuju da mjerenjem koncentracije vodikovog
peroksida, naprednog produkta glikacije i specifične enzimske aktivnosti
katalaze razine su bile niže u homogenatima cijelog tijela kod F1 potomaka
selektiranih sojeva 22. i 28. generacije HP i LP soja u odnosu na kontrolnu
skupinu. Mjerenjem specifične enzimske aktivnosti superoksid dismutaze
(SOD) nisu opažene razlike između F1 potomaka eksperimentalnih skupina,
razlike nije bilo niti u odnosu na kontrolnu skupinu. Kod F2 i F3 potomaka 22.
generacije HP i LP soja nema razlike u mjerenim redoks parametrima, osim u
SOD specifičnoj enzimskoj aktivnosti, dok kod F2 i F3 potomaka 28. generacije
HP i LP soja se svi parametri mijenjaju osim SOD specifične enzimske
aktivnosti.
U ovom eksperimentalnom radu je prikazano da konzumacija METH-a
roditeljske generacije djeluje na promjenu mjerenih redoks parametara kod
potomaka. Nestabilnost u 28. generaciji HP i LP soja ukazuje na potencijalne
epigenetske promjene. Kako one nisu uočene u 22. generaciji pretpostavka je
da je preferencija 22. generacije posljedica obogaćenja genskih varijanti koje
su rezultirale HP i LP fenotipom. Kako bi se ova pretpostavka istražila potrebno
je provesti buduća istraživanja.
Abstract (english) Metamphetamine (METH) is a psychostimulant that is widely abused
around the world. There is an increasing prevalence of addiction that can
potentially be associated with offspring whose parents were addicted, making
the offspring a risk group. METH in the body affects the morphological and
functional changes of neural networks – neural plasticity. Our model for
studying neural plasticity is METH self-administration, which we use to
implement artificial selection to investigate the neurobiological basis of the of
the difference in high (HP) and low (LP) preference.
The aim of this experimental work was to determine whether
preferential METH self-administration will show changes in the redox
parameters of F1 filial generation of the 22nd and 28th generations of HP and
LP strains, and whether these redox parameters will be stable in the F2 and
F3 filial generation of the 22nd and 28th generations of HP and LP strains after
the end of selection.
The obtained results show that by measuring the concentration of
hydrogen peroxide, advanced glycation product and catalase specific enzyme
activity, the levels were lower in the whole body homogenates of the F1 filial
generation of the selected strains of the 22nd and the 28th generations of the
HP and LP strains compared to the control group. By measuring the superoxide
dismutase (SOD) specific enzyme activity, no differences were observed
between the F1 filial generation of the experimental groups, nor was there a
difference compared to the control group. In the F2 and F3 filial generations
of the 22nd generation HP and LP strains, there is no difference in the
measured redox parameters, except for SOD specific enzyme activity, while
in the F2 and F3 filial generations of the 28th generation HP and LP strains, all
parameters change except SOD specific enzyme activity. In this experimental work, it was shown that METH consumption of the
parental generation affects the change of measured redox parameters in the
filial generations. Instability in the 28th generation HP and LP strains indicates
potential epigenetic changes. Since they were not observed in the 22nd
generation, the assumption is that the preference of the 22nd generation is
due to the enrichment of gene variants that resulted in the HP and LP
phenotype. In order to investigate this assumption, it is necessary to conduct
future research.
Keywords
Drosophila melanogaster
redoks parametri
reaktivne kisikove vrste
metamfetamin
preferencija
Keywords (english)
Drosophila melanogaster
redox parameters
reactive oxigen species
metamphetamine
preference
Language croatian
URN:NBN urn:nbn:hr:193:390057
Study programme Title: Drug research and development Study programme type: university Study level: graduate Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resource Text
File origin Born digital
Access conditions Open access
Terms of use
Created on 2023-10-11 12:47:04