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master's thesis
The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster
Rijeka: University of Rijeka, 2023. urn:nbn:hr:193:610593
Fućak, Laura
University of Rijeka
Faculty of Biotechnology and Drug Development

Institutional repository: Repository of the University of Rijeka, Faculty of Biotechnology and Drug Development

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Fućak, L. (2023). The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:610593

Fućak, Laura. "The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:610593

Fućak, Laura. "The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:610593

Fućak, L. (2023). 'The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster', Master's thesis, University of Rijeka, accessed 06 August 2024, https://urn.nsk.hr/urn:nbn:hr:193:610593

Fućak L. The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster [Master's thesis]. Rijeka: University of Rijeka; 2023 [cited 2024 August 06] Available at: https://urn.nsk.hr/urn:nbn:hr:193:610593

L. Fućak, "The effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster", Master's thesis, University of Rijeka, Rijeka, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:193:610593

Metadata
TitleThe effect of preferential methamphetamine consumption on lifespan and behavioral phenotypes of Drosophila melanogaster
Title (croatian)Utjecaj preferencijalne konzumacije metamfetamina na dužinu životnog ciklusa i bihevioralne fenotipove Drosophile melanogaster
AuthorLaura Fućak
MentorRozi Andretić Waldowski (mentor)
MentorAna Filošević Vujnović (komentor)
Committee memberRozi Andretić Waldowski (član povjerenstva)
Committee memberAna Filošević Vujnović (član povjerenstva)
Committee memberAnđelka Radojčić Badovinac (predsjednik povjerenstva)
Committee memberDuško Čakara (član povjerenstva)
Committee memberMiranda Mladinić Pejatović (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2023-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Addiction is a relapsing disease caused by substance abuse. Methamphetamine and cocaine are potent psychostimulants whose abuse induces numerous negative consequences for the abuser. Studying substance abuse in humans is challenging, however its effects can be analyzed in model organisms such as Drosophila melanogaster. Alongside their genetic traceability, fruit flies are extensively researched making them exemplary models for addiction research. Methamphetamine and cocaine disbalance monoaminergic levels, damage axons and create reactive oxygen species which disrupt normal organism functions. Considering that addiction shows around 72% heritability, we were wondering if the effects of paternal methamphetamine abuse can be seen in filial generations. After artificial selection of the 28th Canton S fly generation with high (HP) and low (LP) preference for methamphetamine, we observed life cycle and behavioral phenotypes of flies, their morphology and voluntary self-administration of methamphetamine through FlyCAFE assay. Our aim was to determine if there are any differences between wt, HP and LP lines after 28 generations of selection. The obtained data shows impaired activity, sleep, and negative geotaxis of flies, alongside possible changes in flies weight and size compared to control wt line. Locomotor activity of HP, LP, and wt flies increases after volatilized methamphetamine and cocaine administration with various significances among lines. The HP and LP flies maintain the same preference for methamphetamine, high and low, respectively. Finally, there is no difference in length of life cycle among lines, but HP flies have lower reproduction success rate. Our findings point to a transgenerational effect of paternal methamphetamine abuse, posing further questions about the epigenetic and/or genetic changes induced by substance abuse.
Abstract (croatian)
Ovisnost je relapsirajuća bolest uzrokovana zlouporabom opojnih supstanci. Metamfetamin i kokain su snažni psihostimulansi čija konzumacija ostavlja brojne negativne posljedice na organizam ovisnika. Izučavanje zlouporabe doga u ljudi vrlo je izazovno, stoga se njihovi učinci analiziraju u modelnim organizmima poput vinske mušice (Drosophila melanogaster). Vinske mušice pogodne su za istraživanja ovisnosti jer imaju dobro proučen i sekvenciran genom pa se njihove genetske promjene mogu jednostavnije pratiti i izučavati. Metamfetamin i kokain uzrokuju poremećaje u nivoima monoamina, oštećuju aksone te stvaraju reaktivne kisikove vrste, što otežava razne funkcije organizma. S obzirom na činjenicu da ovisnost pokazuje oko 72% nasljednosti, interesiralo nas je da li se učinci paternalne preferencijalne samo-administracije metamfetamina očituju u filijalnim generacijama mušica. Nakon umjetne selekcije 28. generacije mušica Canton S linije sa visokom (HP) i niskom (LP) preferencijom za metamfetamin, proučavali smo životni ciklus i bihevioralne fenotipove D. melanogaster. Također smo promatrali morfologiju mušica i larvi te samo-administraciju metamfetamina kroz FlyCAFE metodu. Cilj ove teze bio je vidjeti postoje li razlike između wt, HP i LP linija nakon 28 generacija umjetne selekcije. Prikupljeni rezultati ukazuju na povećanu aktivnost i smanjen san mušica, kao i na smanjenu sposobnost negativne geotaksije. Nadalje, postoji mogućnost da visoka i niska paternalna preferencijalna samo-administracija metamfetamina utječe na veličinu i težinu mušica, odnosno larvi u odnosnu na wt. Lokomotorna aktivnost mušica HP, LP i wt linija raste nakon administracije volatiliziranog metamfetamina i kokaina sa raznim nivoima statističke značajnosti unutar linija. HP linija zadržava visoku, a LP linija nisku preferenciju metamfetamina, što je u skladu s prethodno testiranim generacijama. Konačno, nema promjena u duljini životnog ciklusa mušica, no HP linija ima nižu reprodukcijsku uspješnost. Navedeni rezultati ukazuju na trans-generacijski efekt paternalne preferencijalne samo-administracije metamfetamina, te postavljaju pitanja o potencijalnim epigenetskim ili genetskim promjenama koje su inducirane zlouporabom supstanci.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:610593
Study programmeTitle: Medicinal chemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinske kemije (magistar/magistra medicinske kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-27 14:09:52