This doctoral dissertation deals with practical artistic research, analysis and creative application of tools, methods and techniques of acting in theatre, film and radio media. The doctoral dissertation is based on extensive artistic research within which Kordelija Paladin’s monodrama Jedna od sestara has been staged in three different artistic media: as a movie in the virtual reality medium, as a theatre performance and as a radio drama. Being a transparent theatre form, monodrama provided the opportunity for research and practical use of dramatic play models and purposeful models of dramatic play and communication from three acting systems of Antonin Artaud, Bertolt Brecht and Konstantin S. Stanislavsky, as well as acting methods of Mikhail Chekhov, Sanford Meisner and Boro Stjepanović. The artistic part of this dissertation presents practical work for the purpose of synthetizing the results of the practical application of acting tools from the mentioned, well-known acting systems and methods. The doctoral dissertation describes all these processes, as well as the process that preceded the artistic part of the doctoral research, the transformation of an idea into the dramatic text and the cooperation between the actress and the playwriter that resulted in the monodrama Jedna od sestara, created specifically for the purpose of this doctoral research. The textual template has been used to test the following acting models: actor in front of the camera, actor in front of the audience, and actor in front of the microphone. The acting tools that the actress chose in cooperation with her mentor and colleagues have been examined practically during the development of the artistic act within different performing and artistic forms. All the above stated has been tested on several occasions in front of the audience. After 11 theatrical performances of the monodrama, the audience was asked to fill out a survey on the models of the play and the communication with the audience. The results of the survey have been processed and described in the final part of this dissertation. The hypothesis that acting is an authentic and organic art that “co-writes” and fills in the spaces of literature, directing, scenography and multimedia “emptiness” in media has been defended by the actress through her acting and organic engagement in the artistic work. The written part of the doctoral dissertation is accompanied by the appendices: the recording of the play, VR movie, radio drama, newspaper articles, reviews and TV reports on the play. The research and acting preparation required for different models of acting adapted to different media has been synthesised and presented in tables.