Theoretical part of this paper, stresses the key characteristics of work motivation, job satisfaction for three different and work active generations, which are described in the paper. Important concepts are discussed, and each generation is separately researched. The included generations represent the current workforce in the market, and those are: Generation X, Generation Y (Millennials) and only a part of Generation Z, due to fact that the majority of this generation is still in the education system. Each generation has different needs in the work relationship, resulting in different satisfying motivational factors. The research aims to identify similarities and differences in motivation and job satisfaction among the three mentioned generations. Each satisfied and motivated employee contributes to the success of the company and represents its’ important part. The second part of the work is empirical in nature, where an anonymous survey consisting of 25 questions was conducted, with 161 respondents providing their answers. Based on the sample results, specific conclusions on the topic are presented. The analysis confirms the relations with the characteristics discussed in the first part and highlights less significant differences. The most notable motivational factor across all generations is the financial factor. Regarding job satisfaction, the question related to satisfaction with colleagues in the workplace stood out with the highest satisfaction, while the dissatisfaction was the most pronounced in terms of advancement opportunities in the job.