Mass tourism and its influence on the development of tourist destinations are analyzed in this master thesis on the example of Venice. Tourism is considered as an important factor in local economic development, but its rapid growth can negatively affect the quality of life for the local community, destroy the environment and heritage, but also change some other economic, social and ecological aspects. This thesis aims to analyze sustainable development strategies in order to mitigate mass tourism consequences. To write this thesis, secondary research has been conducted. The analyzed data includes publications from international organizations, tourist organizations, and other scientific articles and internet sources. Nowadays, Venice is an important international tourist destination in which tourism plays a significant role in economic growth. On the other hand, the pressure of tourism development (especially day trips) can degrade the quality of Venice as a tourist destination. Local authorities have an important part in the environment and cultural heritage protection, but also in raising awareness about the need for such type of protection, i.e. the need for sustainable tourism development. But Venice is also an example of a destination in which local authorities are not doing enough in tourism development and are not managing their visitors well. Today, there are more tourists in the historic center of Venice than there are residents. The tourists, unlike the residents, are not emotionally attached to the city and its history, so there will always exist a conflict between the locals’ needs and the tourists’ needs. This situation requires appropriate measures of sustainable development.