The business world has faced a major problem in the past year due to the pandemic caused by the COVID-19 virus, which brought various extraordinary circumstances and numerous challenges, and required managers and employees to adapt to new ways of dealing with stress. In such circumstances, an imbalance between different requirements of managers and the ability of employees to fulfill these requirements can affect the occurrence of stress in employees, which over time can have severe consequences for employees and for the entire organization. The importance of managers' emotional intelligence comes to the fore here because it contains social and emotional skills that are crucial in recognizing and effectively responding to the various stressful situations faced by their subordinates. The aim of the thesis is to explain and define the concepts of stress and emotional intelligence, and determine the relationship between them, that is the relationship of emotional intelligence of managers measured through 4 dimensions of emotional intelligence and perceived stress levels in new business conditions during the pandemic caused by COVID-19 virus. The research was conducted on a sample of 164 employees, i.e. persons who were employed during the COVID-19 pandemic, using already existing inventories such as the scale of emotional intelligence – WLEIS, LAL1 questionnaire of emotional intelligence and occupational stress index – OSI. According to the results of the research, it can be concluded that the respondents feel average and slightly above-average stress in the new business conditions during the pandemic caused by the COVID-19 virus. Also, it can be concluded that there is a moderately strong and negative association between the emotional intelligence of immediate superiors and perceived stress in employees in the workplace during a pandemic caused by the COVID-19 virus.