The aim of this graduate thesis was to examine the existence of vocal fatigue among catholic priests in Croatia using the Vocal Fatigue Index questionnaire (VFI-C) and compare their results with those of nonprofessional voice users. The study involved a total of 104 participants, 52 priests, and 52 nonprofessional voice users, ages 26 to 65. All participants were male. The subjective voice assessment method, self-assessment questionnaire Vocal Fatigue Index, was used as a measuring instrument. All participants answered questions about general information and previous voice problems, while the group of priests also answered additional questions related to lifestyle habits and environmental and health risk factors. Statistical analysis revealed a statistically significant difference between the group of priests and the group of nonprofessional voice users in the total score on the Vocal Fatigue Index, as well as in all individual questionnaire categories. Priests had a higher number of points in all categories of the Vocal Fatigue Index compared to nonprofessional voice users . There was no statistically significant difference found between priests who consume tobacco products and those who dont. Additionally, no statistically significant correlation was found between the age variable of the priests and the overall result on the VFI-C questionnaire. The results showed that a large majority of the priests did not receive any information about vocal hygiene during their education. All participants agreed that it would be beneficial to acquire such knowledge. Considering that this study has shown that priests perceive symptoms of vocal fatigue at a statistically significant higher level compared to nonprofessional voice useres, and that they lack knowledge about vocal hygiene, it would be beneficial to educate them about ways to maintain the health of their voice.