People use their voice every day to interact with their surroundings. With all the present technology, their voices are still the most important and necessary means of communication. The aim of this study is to investigate whether there is a change in the voice quality of the preschool teachers in the analyzed parameters at the beginning and end of the working day, and to determine whether there are statistically significant differences between the two measurements. Ten preschool teachers of the Duga kindergarten in Zagreb, participated in the research. For research purposes, the preschool teachers filled out a questionnaire on eating habits, years of service, smoking, voice problems, and Vocal FatigueQuestionnairewhich is designed for the research. With a digital recorder, I recorded the vocal phonation /a/ and counting from one to ten, at the beginning and at the end of the working day. Preschool teachers who are practicing some sports (fitness or yoga)have better breathing capacities. Their voices are more durable and stronger. Most preschool teachers consume more than one coffee and cigarette during the day, which contributes to poor quality of voice. A small number of educators consume water during the working day, possibly only once or twice during the day, which is too low in terms of voice use. Many preschool teachers are completely unaware of the importance of vocal hygiene and voice preservation. Results after statistical analysis did not show statistically significant differences between the variables before and after the work. Analyzing the answers of the Vocal Fatigue Questionnaire it is evident that almost all preschool teachers sometimes feel some of the vocal fatigue symptoms. Analysis confirmed the existence of significant correlations between the Questionnaire variables, as well as with some life habits and symptoms of vocal fatiguesmoking for example. This research emphasizes the importance of raising awareness of the preservation of voice within a group of preschool teachers.